高级搜索

低磁场核磁共振对小鼠原发性和移植性肿瘤的成像效果

赵明媛, 高文婷, 宋雅楠, 王福金, 王爱国, 王靖宇

赵明媛, 高文婷, 宋雅楠, 王福金, 王爱国, 王靖宇. 低磁场核磁共振对小鼠原发性和移植性肿瘤的成像效果[J]. 肿瘤防治研究, 2015, 42(11): 1100-1103. DOI: 10.3971/j.issn.1000-8578.2015.11.009
引用本文: 赵明媛, 高文婷, 宋雅楠, 王福金, 王爱国, 王靖宇. 低磁场核磁共振对小鼠原发性和移植性肿瘤的成像效果[J]. 肿瘤防治研究, 2015, 42(11): 1100-1103. DOI: 10.3971/j.issn.1000-8578.2015.11.009
shu
ZHAO Mingyuan, GAO Wenting, SONG Ya'nan, WANG Fujin, WANG Aiguo, WANG Jingyu. Imaging Effect of Low-field MRI on Primary Tumors and Transplanted Tumors in Mice[J]. Cancer Research on Prevention and Treatment, 2015, 42(11): 1100-1103. DOI: 10.3971/j.issn.1000-8578.2015.11.009
Citation: ZHAO Mingyuan, GAO Wenting, SONG Ya'nan, WANG Fujin, WANG Aiguo, WANG Jingyu. Imaging Effect of Low-field MRI on Primary Tumors and Transplanted Tumors in Mice[J]. Cancer Research on Prevention and Treatment, 2015, 42(11): 1100-1103. DOI: 10.3971/j.issn.1000-8578.2015.11.009
shu

低磁场核磁共振对小鼠原发性和移植性肿瘤的成像效果

  • 116044 大连，大连医科大学实验动物中心

基金项目: 国家自然科学基金（30872950）
详细信息
    作者简介:

    赵明媛（1989-），女，硕士在读，主要从事肿瘤活体成像的研究

    通讯作者:

    王爱国，E-mail: wangaiguotl@hotmail.com

    王靖宇，E-mail: wangjingyus@163.com

  • 中图分类号: R730.44

Imaging Effect of Low-field MRI on Primary Tumors and Transplanted Tumors in Mice

  • Laboratory Animal Center, Dalian Medical University, Dalian 116044, China

HTML全文

    摘要
  • 摘要: 目的 利用小鼠原发性和移植性肿瘤模型检测0.5T低磁场小动物核磁共振（MRI）的成像效果。方法 采用0.5T低磁场MRI仪对小鼠皮下移植瘤（HepG2、S180、H-ras12V转基因小鼠肝肿瘤组织）和原发性肝肿瘤（H-ras12V转基因肝癌小鼠）进行检测，观察其影像学表现并对H-ras12V转基因小鼠原发性肝肿瘤进行病理学确认。结果 皮下移植瘤小鼠（S180、HepG2、H-ras12V转基因小鼠肝肿瘤组织）的肿瘤生长状态良好，肉眼及X光检测均可观察到显著的肿瘤团块。MRI成像结果表明，皮下肿瘤部位的T1WI上表现为低信号、T2WI上表现为高信号、成像清晰、效果良好，且三种小鼠皮下移植瘤的MRI成像效果相似。对于H-ras12V转基因小鼠原发性肝肿瘤的MRI成像结果表明，在转基因小鼠的肝区出现了T1WI上表现为低信号的多个区域，这些相应区域在T2WI上表现为高信号。病理解剖及病理切片证实，这些区域为多发性肝肿瘤。结论 利用低磁场MRI可以对小鼠原发性肝肿瘤和移植性肿瘤进行有效的检测，可作为小鼠肿瘤无创检测的有效方法。

     

    Abstract: Objective To investigate the imaging effect of 0.5T low-field MRI on mice harboring primary liver tumors and subcutaneously transplanted tumors. Methods The 0.5T low-field MRI was conducted on mice bearing HepG2, S180, and liver tumor tissues of H-ras12V transgenic mice and primary liver tumors(Hras12V transgenic mice). The primary liver tumors of H-ras12V transgenic mice were confirmed by histopathological examination. Results The subcutaneously transplanted tumors (HepG2, S180, liver tumor tissues of H-ras12V transgenic mice) grew well and the tumor masses could be clearly observed by naked eyes and X optical detection. The MRI imaging results showed that the location of transplanted tumors clearly presented hypointense signal on T1WI and hyperintense signal on T2WI. The MRI imaging manifestations of three kinds of subcutaneously transplantated tumors were consistent. For the H-ras12V transgenic mice, the MRI imaging results showed that several separated regions presented hypointense signal on T1WI and hyperintense signal on T2WI in the location of liver. These regions were finally confirmed to be multiple liver tumors by anatomy and histopathological examination. Conclusion The low-field MRI provides an effective and noninvasive diagnostic method for detecting primary liver tumor and transplanted tumor in mice.

     

    • HTML全文
    • 参考文献(19)
  • [1] Li DM, Wang CL, Li JC. Development of small living animal imaging technology[J]. Zhongguo Sheng Wu Yi Xue Gong Cheng Xue Bao, 2009, 28(6): 916-21. [李冬梅, 万春丽, 李继承. 小动 物活体成像技术研究进展[J]. 中国生物医学工程学报, 2009, 28 (6): 916-21.]
    [2] Sun JJ, He XB, Bu YS. Research on the layout and construction preparation for 3.0T high-field nuclear magnetic resonance equipment room[J]. Zhongguo Yi Xue Zhuang Bei, 2013, 10(10): 53 -5. [孙继江, 何晓彬, 步岩生. 3.0T高场强核磁共振设备机房 布局及施工准备[J]. 中国医学装备, 2013, 10(10): 53-5.]
    [3] Wang HT, Zhao HZ, Fu YF, et al. The low field mri diagnosis of brain arteriovenous fistula, and the evaluation of the difference between high and low field nuclear magnetic resonance (NMR)[J]. Zhongguo Zhong Yi Yao Xian Dai Yuan Cheng Jiao Yu, 2012, 10 (9): 105, 110. [王海涛, 赵汉卓, 付云峰, 等. 脑动静脉畸形的 低场磁共振诊断及高低场核磁共振的区别评价[J]. 中国中医药 现代远程教育, 2012, 10(9): 105, 110.]
    [4] Cao Y, Liu MY, Liu B, et al. The value of low-field MRI in the diagnosis and staging of cervial cancer[J]. Yi Xue Zong Shu, 20 12, 18(15): 2507-8. [曹阳, 刘茂华, 柳彬. 低场MRI在宫颈癌 诊断及分期中的应用价值[J]. 医学综述, 2012, 18(15): 2507-8.]
    [5] Song GJ, Zheng LY, Yang HY. The diagnostic analysis of 40 of low field MRI in breast tumors[J]. Zhongguo Wu Zhen Xue Za Zhi, 2011, 11(28): 6962-3. [宋国军, 郑来煜, 杨海燕. 低场MRI 在乳腺肿块诊断中的应用40例分析[J]. 中国误诊学杂志, 2011, 11 (28): 6962-3.]
    [6] Fu DY, Wang CD, Li SK, et al. Clinical study of low-field magnetic resonance imaging in diagnosis of early cerebral hemorrhage[J]. Zhongguo Yi Yao Ke Xue, 2013, 3(7): 127-9, 140. [符大勇, 王长德, 李士坤, 等. 低场核磁共振诊断早期脑出血的 临床研究[J]. 中国医药科学, 2013, 3(7): 127-9, 140.]
    [7] Hosui A, Kimura A, Yamaji D, et al. Loss of STAT5 causes liver fibrosis and cancer development through increased TGF-{beta} and STAT3 activation[J]. J Exp Med, 2009, 206(4): 819-31.
    [8] Zhang G. High field MRI system of knee joint imaging technology and clinical application[J]. Heilongjiang Yi Yao Ke Xue, 2013, 36(1): 106. [张刚. 高场核磁共振系统膝关节成像技 术及临床应用[J]. 黑龙江医药科学, 2013, 36(1): 106.]
    [9] Lee RK, Griffith JF, Lau YY, et al. Diagnostic capability of low- versus high-field magnetic resonance imaging for lumbar degenerative disease[J]. Spine (Phila Pa 1976), 2015, 40(6): 38 2-91.
    [10] Huang LG. Clinical analysis of lowfield magnetic resonance imaging in diagnosis of early cerebral hemorrhag[J]. Zhong Wai Jian Kang Wen Zhai, 2013, 10(36): 155-6. [黄龙国. 低场核磁共 振诊断早期脑出血的临床分析[J]. 中外健康文摘, 2013, 10(36): 15 5-6.]
    [11] Santos MP, Gutierrez-Nibeyro SD, McKnight AL, et al. Gross and his topathologic correlation of low-field magnetic resonance imaging findings in the stifle of asymptomatic horses[J]. Vet Radiol Ultrasound, 2015, 56(4):407-16.
    [12] Ley CJ, Björnsdóttir S, Ekman S, et al. Detection of early osteoarthritis in the centrodistal joints of Icelandic horses: Evaluation of radiography and low-field magnetic resonance imaging[J]. Equine Vet J, 2014, [Epub ahead of print].
    [13] Tamura S, Doi S, Tamura Y, et al. Thoracolumbar intradural disc herniation in eight dogs: clinical, low-field magnetic resonance imaging, and computed tomographic myelography findings[J]. Vet Radiol Ultrasound, 2015, 56(2): 160-7.
    [14] Sun J, Zhang XP, Li XT, et al. Applicable apparent diffusion coefficient of an orthotopic mouse model of gastric cancer by improved clinical MRI diffusion weighted imaging[J]. Sci Rep, 20 14, 4: 6072.
    [15] Wang F, Jiang R, Takahashi K, et al. Longitudinal assessment of mouse renal injury using high-resolution anatomic and magnetization transfer MR imaging[J]. Magn Reson Imaging, 20 14, 32(9): 1125-32.
    [16] Xue JT, Zhu P, Zhao XF. Basic hospital application of low field magnetic resonance significance[J]. Xin Li Yi Sheng, 2010, 20 10(7): 29-30. [薛江涛, 朱萍, 赵序芳. 基层医院运用低场强磁 共振的意义[J]. 心理医生, 2010, 2010(7): 29-30.]
    [17] Jiang HF, Ren J, Cui Y, et al. Establishment of an orlhotropic tumor model for hepatocellular carcinoma and non-invasive in vivo tumor imaging by MRI in mice[J]. Xian Dai Zhong Liu Yi Xue, 2007, 15(2): 174-6. [姜晗昉, 任军, 崔勇, 等. 小鼠原位移 植性肝癌模型的建立及其磁共振表现[J]. 现代肿瘤医学, 2007, 15 (2): 174-6.]
    [18] Wang YF. Hepatocellular carcinoma in elderly patients with propofol and isoflurane anesthesia comparison[J]. Zhongguo Lao Nian Xue Za Zhi, 2012, 32(10): 2151-2. [王允飞. 老年肝癌患 者应用丙泊酚和异氟烷麻醉对比[J]. 中国老年学杂志, 2012, 32 (10): 2151-2.]
    [19] Liu T, Zhang RM. Isoflurane inhalation anesthesia combining with propofol intravenous anesthesia for laparoscopic hysterectomy effect[J]. Zhongguo Lao Nian Xue Za Zhi, 2013, 33(19): 4737-9. [刘涛, 张荣明. 异氟烷吸入麻醉复合丙泊酚静脉麻醉用于腹 腔镜子宫切除术的效果[J]. 中国老年学杂志, 2013, 33(19): 47 37-9.]
    • 相关文章
    • 施引文献
    • 资源附件(0)
计量
  • 文章访问数:  1498
  • HTML全文浏览量:  310
  • PDF下载量:  634
  • 被引次数: 0
出版历程
  • 收稿日期:  2014-11-13
  • 修回日期:  2015-06-28
  • 刊出日期:  2015-11-24

目录

摘要
HTML全文
    参考文献(19)
    相关文章
    施引文献
    资源附件(0)

    /

    返回文章
    返回

    下载中心

    友情链接

    联系我们

    地址：武汉市洪山区卓刀泉南路116号（430079）

    电话：027－87670126

    Email：zlfzyjzz@vip.163.com

    This work is licensed under a Creative Commons Attribution 3.0 License.

    邮件订阅 RSS
    总访问量：12497969 今日访问：14740

    版权所有 © 《肿瘤防治研究》编辑部 鄂公网安备 42011102005013号 鄂ICP备2022015867号

    本系统由北京仁和汇智信息技术有限公司开发  百度统计

    x 关闭 永久关闭