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热疗降低胶质瘤侵袭性及与HSP70和iNOS表达的关系

张田, 贾永森, 孙月明, 彭海兵, 秦丽娟, 王银环

张田, 贾永森, 孙月明, 彭海兵, 秦丽娟, 王银环. 热疗降低胶质瘤侵袭性及与HSP70和iNOS表达的关系[J]. 肿瘤防治研究, 2015, 42(11): 1063-1066. DOI: 10.3971/j.issn.1000-8578.2015.11.001
引用本文: 张田, 贾永森, 孙月明, 彭海兵, 秦丽娟, 王银环. 热疗降低胶质瘤侵袭性及与HSP70和iNOS表达的关系[J]. 肿瘤防治研究, 2015, 42(11): 1063-1066. DOI: 10.3971/j.issn.1000-8578.2015.11.001
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ZHANG Tian, JIA Yongsen, SUN Yueming, PENG Haibing, QIN Lijuan, WANG Yinhuan. Effect of HSP70 and iNOS in Process of Hyperthermia Reducing Glioma Invasion[J]. Cancer Research on Prevention and Treatment, 2015, 42(11): 1063-1066. DOI: 10.3971/j.issn.1000-8578.2015.11.001
Citation: ZHANG Tian, JIA Yongsen, SUN Yueming, PENG Haibing, QIN Lijuan, WANG Yinhuan. Effect of HSP70 and iNOS in Process of Hyperthermia Reducing Glioma Invasion[J]. Cancer Research on Prevention and Treatment, 2015, 42(11): 1063-1066. DOI: 10.3971/j.issn.1000-8578.2015.11.001
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热疗降低胶质瘤侵袭性及与HSP70和iNOS表达的关系

  • 1. 063000 唐山，华北理工大学基础医学院；2. 063000 唐山，华北理工大学中医学院；3. 063000 唐山，华北理工大学冀唐学院

基金项目: 河北省中医药管理局中医药类科研项目（2014195）；河北省卫生厅科研基金项目（20150491）
详细信息
    作者简介:

    张田（1979-），女，硕士，讲师，主要从事脑肿瘤治疗相关研究

    通讯作者:

    秦丽娟，E-mail：qinlj20012003@163.com

  • 中图分类号: R739.41

Effect of HSP70 and iNOS in Process of Hyperthermia Reducing Glioma Invasion

  • 1. School of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063000, China; 2. College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan 063000, China; 3. Jitang College of North China University of Science and Technology, Tangshan 063000, China

摘要

    摘要
  • 摘要: 目的 探讨热疗与热休克蛋白70（heat shock protein70, HSP70）及诱导型一氧化氮合酶（induced nitric oxide synthase, iNOS）表达的关系，阐明热疗降低胶质瘤侵袭性的作用及其可能机制。方法 热处理C6胶质瘤细胞后，酶联免疫吸附法和Western blot法动态检测胶质瘤细胞中HSP70抗体滴度和iNOS的表达水平；Griess法测定胶质瘤细胞中NO浓度；结晶紫染色法检测胶质瘤侵袭性的改变。结果 C6细胞经热疗后，HSP70抗体滴度增加，于30 min时最多（86.52 ng/L）。C6细胞内iNOS的表达水平也在热疗后明显增加，并于热疗60 min达高峰（IDV=228.31）后逐渐减少。热疗后胶质瘤细胞内NO浓度于热疗120 min时达高峰（OD=168.42），且NO浓度越高，胶质瘤侵袭性越低（n=8.21×106）。结论 胶质瘤细胞经热疗后HSP70表达水平增加，增加的HSP70可能通过某种机制提高了iNOS的表达水平，进而增加了胶质瘤细胞内的NO浓度而引起胶质瘤侵袭性的下降。

     

    Abstract: Objective To explore the relationship of hyperthermia with heat shock protein 70(HSP70) and induced nitric oxide synthase(iNOS) expression and to clarify hyperthermia reducing glioma invasiveness and its possible mechanism. Methods After heat treatment on glioma cells C6, we determined HSP70 antibody titer and the expression level of iNOS by ELISA and Western blot; Nitric oxide(NO) concentration in glioma cells were determined by Griess; We used crystal violet staining method to determine the glioma invasion. Results HSP70 antibody titer in C6 cells was increased and reached the maximum at 30 min(86.52ng/L) after hyperthermia. The expression level of iNOS in C6 cells was also increased significantly and reached the peak at 60min(IDV=228.31) after hyperthermia, then gradually decreased. NO concentration of glioma cells reached the peak at 120min(OD=168.42) after hyperthermia. The higher NO concentration was, the lower glioma invasion was(n=8.21×106). Conclusion Hyperthermia increases HSP70 expression in glioma cells. HSP70 probably improves the expression level of iNOS by a mechanism, thereby increases the concentration of NO in glioma cells and decreases the glioma invasion.

     

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出版历程
  • 收稿日期:  2014-11-13
  • 修回日期:  2015-03-12
  • 刊出日期:  2015-11-24

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