高级搜索

食管鳞癌中IDO的表达及其与肿瘤血管形成的关系

王郁, 贾云泷, 王婷婷, 王淼, 段玉青, 王洪琰, 孟宪利, 刘丽华

王郁, 贾云泷, 王婷婷, 王淼, 段玉青, 王洪琰, 孟宪利, 刘丽华. 食管鳞癌中IDO的表达及其与肿瘤血管形成的关系[J]. 肿瘤防治研究, 2015, 42(09): 892-896. DOI: 10.3971/j.issn.1000-8578.2015.09.007
引用本文: 王郁, 贾云泷, 王婷婷, 王淼, 段玉青, 王洪琰, 孟宪利, 刘丽华. 食管鳞癌中IDO的表达及其与肿瘤血管形成的关系[J]. 肿瘤防治研究, 2015, 42(09): 892-896. DOI: 10.3971/j.issn.1000-8578.2015.09.007
WANG Yu, JIA Yunlong, WANG Tingting, WANG Miao, DUAN Yuqing, WANG Hongyan, MENG Xianli, LIU Lihua. IDO Expression in Esophageal Squamous Cell Carcinoma and Its Relationship with Tumor Angiogenesis[J]. Cancer Research on Prevention and Treatment, 2015, 42(09): 892-896. DOI: 10.3971/j.issn.1000-8578.2015.09.007
Citation: WANG Yu, JIA Yunlong, WANG Tingting, WANG Miao, DUAN Yuqing, WANG Hongyan, MENG Xianli, LIU Lihua. IDO Expression in Esophageal Squamous Cell Carcinoma and Its Relationship with Tumor Angiogenesis[J]. Cancer Research on Prevention and Treatment, 2015, 42(09): 892-896. DOI: 10.3971/j.issn.1000-8578.2015.09.007

食管鳞癌中IDO的表达及其与肿瘤血管形成的关系

基金项目: 国家自然科学基金(81201607);河北省杰出青年基金(H2014206320);河北省自然科学基金(H2012206135)
详细信息
    作者简介:

    王郁(1987-),女,硕士,主要从事肿瘤生物治疗的基础研究与临床治疗工作

    通讯作者:

    刘丽华,E-mail:lihualiu567@hotmail.com

  • 中图分类号: R735.1

IDO Expression in Esophageal Squamous Cell Carcinoma and Its Relationship with Tumor Angiogenesis

  • 摘要: 目的 检测吲哚胺2,3-双加氧酶(Indoleamine 2,3-dioxygenase, IDO)在人食管鳞癌及其癌旁组织中的表达及规律,通过检测VEGF的表达探索IDO及肿瘤血管形成的相关性,并了解两者在食管鳞癌的进展及转移中的作用。方法 收集56例河北医科大学第四医院行食管切除患者的肿瘤组织及其癌旁组织,使用半定量RT-PCR法和免疫组织化学法检测组织样本中IDO和VEGF的mRNA和蛋白表达情况,并分析在食管鳞癌组织中IDO与肿瘤血管形成的相关性。结果 与癌旁组织相比,食管鳞癌组织中的IDO和VEGF阳性表达率和表达水平均显著升高(P=0.020,P=0.022),其表达与患者年龄、性别等无关(P>0.05),与患者TNM分期、肿瘤侵犯深度、分化程度、淋巴结转移相关(P<0.05)。食管鳞癌组织中IDO的表达与VEGF的表达呈显著正相关(r=0.533,P=0.001)。结论 在食管鳞癌中IDO可能与肿瘤血管的形成有关。

     

    Abstract: Objective To test the expression of IDO and VEGF mRNA in tumor tissues and para-carcinoma tissues of esophageal squamous cell carcinoma(ESCC)patients and to investigate their associations with tumor invasion and lymphatic metastasis. Methods Carcinoma tissue and para-carcinoma tissue specimens were collected from 56 cases of ESCC patients who underwent esophagectomy in The Fourth Hospital of Hebei Medical University. Optical density semi-quantitative RT-PCR and immunohistochemical method were used to detect the expression of IDO and VEGF mRNA. The association between the expressions of IDO, VEGF mRNA and patients' clinical features were analyzed. Results Compared with para-carcinoma tissues, the expression levels of IDO and VEGF mRNA in ESCC tissues were significantly increased (P=0.020, P=0.022). The expression of IDO and VEGF mRNA were both associated with patients' TNM stage, tumor invasion depth, tumor differentiation and lymph node metastasis (P<0.05), but age or gender(P>0.05). Meanwhile the expression of IDO mRNA was positively correlated with VEGF mRNA significantly (r=0.533, P=0.001). Conclusion In ESCC tissues, the expression of IDO and VEGF mRNA are significantly up-regulated. These changes of gene expressions are associated with patients' clinical features, which indicate that they might play a key role in tumor invasion and lymph node metastasis.

     

  • [1] Baba Y, Watanabe M, Shigaki H, et al. Negative lymph-node count is associated with survival in patients with resected esophageal squamous cell carcinoma[J]. Surgery, 2013, 153(2): 23 4-41.
    [2] Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation[J]. Cell, 2011, 144(5): 646-74.
    [3] Li T, Yang Y, Hua X, et al. Hepatocellular carcinoma-associated fibroblasts trigger NK cell dysfunction via PGE2 and IDO[J].Cancer Lett, 2012, 318(2): 154-61.
    [4] Uyttenhove C, Pilotte L, Théate I, et al. Evidence for a tumoral immune resistance mechanism based on tryptophan degradation by indoleamine 2,3-dioxygenase[J]. Nat Med, 2003, 9(10): 12 69-74.
    [5] Wainwright DA, Balyasnikova IV, Chang AL, et al. IDO expression in brain tumors increases the recruitment of regulatory T cells and negatively impacts survival[J]. Clin Cancer Res, 2012, 18 (22): 6110-21.
    [6] Mellor AL, Munn DH. Physiologic control of the functional status of Foxp3+ regulatory T cells[J]. J Immunol, 2011, 186(8): 45 35-40.
    [7] Sharma MD, Hou DY, Baban B, et al. Reprogrammed foxp3(+) regulatory T cells provide essential help to support crosspresentation and CD8(+) T cell priming in naive mice[J]. Immunity, 2010, 33(6): 942-54.
    [8] Spranger S, Spaapen RM, Zha Y, et al. Up-regulation of PD-L1, IDO, and T(regs) in the melanoma tumor microenvironment is driven by CD8(+) T cells[J]. Sci Transl Med, 2013, 5(200): 20 0ra116.
    [9] Gao YF, Peng RQ, Li J, et al. The paradoxical patterns of expression of indoleamine 2,3-dioxygenase in colon cancer[J]. J Transl Med, 2009, 7: 71.
    [10] Marti LC, Pavon L, Severino P, et al. Vascular endothelial growth factor-A enhances indoleamine 2,3-dioxygenase expression by dendritic cells and subsequently impacts lymphocyte proliferation[J]. Mem Inst Oswaldo Cruz, 2014, 109(1): 70-9.
    [11] Giatromanolaki A, Bates GJ, Koukourakis MI, et al. The presence of tumor-infiltrating FOXP3+ lymphocytes correlates with intratumoral angiogenesis in endometrial cancer[J]. Gynecol Oncol, 2008, 110(2): 216-21.
    [12] Terme M, Pernot S, Marcheteau E, et al. VEGFA-VEGFR pathway blockade inhibits tumor-induced regulatory T-cell proliferation in colorectal cancer[J]. Cancer Res, 2013, 73(2): 53 9-49.
    [13] Knies-Bamforth UE, Fox SB, Poulsom R, et al. c-Myc interacts with hypoxia to induce angiogenesis in vivo by a vascular endothelial growth factor-dependent mechanism[J]. Cancer Res, 20 04, 64(18): 6563-70.
计量
  • 文章访问数:  1451
  • HTML全文浏览量:  355
  • PDF下载量:  102414
  • 被引次数: 0
出版历程
  • 收稿日期:  2014-09-21
  • 修回日期:  2015-04-09
  • 刊出日期:  2015-08-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭