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大肠癌中Pokemon的表达及与前哨淋巴结微转移的关系

王鹏飞, 王涛, 黄晓俊, 王祥, 张德奎, 樊红, 冯彦虎, 王伟, 吴静

王鹏飞, 王涛, 黄晓俊, 王祥, 张德奎, 樊红, 冯彦虎, 王伟, 吴静. 大肠癌中Pokemon的表达及与前哨淋巴结微转移的关系[J]. 肿瘤防治研究, 2015, 42(06): 587-590. DOI: 10.3971/j.issn.1000-8578.2015.06.012
引用本文: 王鹏飞, 王涛, 黄晓俊, 王祥, 张德奎, 樊红, 冯彦虎, 王伟, 吴静. 大肠癌中Pokemon的表达及与前哨淋巴结微转移的关系[J]. 肿瘤防治研究, 2015, 42(06): 587-590. DOI: 10.3971/j.issn.1000-8578.2015.06.012
WANG Pengfei, WANG Tao, HUANG Xiaojun, WANG Xiang, ZHANG Dekui, FAN Hong, FENG Yanhu, WANG Wei, WU Jing. Relationship Between Pokemon Expression and Sentinel Lymph Node Micro-metastasis in Colorectal Cancer[J]. Cancer Research on Prevention and Treatment, 2015, 42(06): 587-590. DOI: 10.3971/j.issn.1000-8578.2015.06.012
Citation: WANG Pengfei, WANG Tao, HUANG Xiaojun, WANG Xiang, ZHANG Dekui, FAN Hong, FENG Yanhu, WANG Wei, WU Jing. Relationship Between Pokemon Expression and Sentinel Lymph Node Micro-metastasis in Colorectal Cancer[J]. Cancer Research on Prevention and Treatment, 2015, 42(06): 587-590. DOI: 10.3971/j.issn.1000-8578.2015.06.012

大肠癌中Pokemon的表达及与前哨淋巴结微转移的关系

详细信息
    作者简介:

    王鹏飞(1982-),男,硕士,主治医师,主要从事消化道肿瘤的研究

    通讯作者:

    吴静,E-mail: wujing36@163.com

  • 中图分类号: R735. 3+4

Relationship Between Pokemon Expression and Sentinel Lymph Node Micro-metastasis in Colorectal Cancer

  • 摘要: 目的 探索大肠癌中Pokemon的表达与大肠癌前哨淋巴结微转移的关系。方法 采用RT-PCR方法检测58例Dukes A-B期大肠癌组织中癌巢、癌以远组织中Pokemon mRNA的表达水平。采用免疫组织化学法检测其中56例大肠癌癌巢中Pokemon蛋白和前哨淋巴结CK20蛋白的表达。 结果 大肠癌癌巢中Pokemon mRNA水平显著高于癌以远组织(P=0.000)。Pokemon蛋白阳性与阴性患者前哨淋巴结发生微转移的比例间差异未达统计学意义(P=0.562)。Pokemon蛋白阳性的患者中CK20阴性的患者生存率高于CK20阳性的患者(P=0.039)。结论 Pokemon在大肠癌癌巢中高表达,联合检测Pokemon与前哨淋巴结CK20在大肠癌预后判断中有重要价值,但尚不能认为Pokemon蛋白可以影响大肠癌前哨淋巴结微转移。

     

    Abstract: Objective To investigate the relationship between the expression of POK erythroid ontogenic factor(Pokemon) and sentinel lymph node micro-metastasis(SLNMM) in colorectal cancer. Methods The expression of Pokemon mRNA in the tumor and surrounding tissues were detected by reverse transcription polymerase chain reaction(RT-PCR) from 58 cases of stage Dukes A-B colorectal cancer cured by operation. The expression of Pokemon protein and sentinel lymph node cytokeratin20(CK20) protein were detected by immunohistochemical(IHC) techniques in colorectal cancer tissues. Results The expression of Pokemon mRNA in colorectal cancer tissues was markedly higher than that in the surrounding tissues(P=0.000) . But the rates of micro-metastasis between the groups of positive and negative Pokemon expression had no significant difference(P=0.562), and it was shown that the survival rate of patients with Pokemon(+)CK20(-) expression was higher than that of those with Pokemon(+)CK20(+) expression(P=0.039). Conclusion Pokemon is overexpressed in colorectal cancer tissues. The combined detection of Pokemon and sentinel lymph node CK20 protein has important value in the prognosis of colorectal cancer. But we could not consider that Pokemon protein could affect SLNMM yet.

     

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出版历程
  • 收稿日期:  2014-06-21
  • 修回日期:  2014-12-11
  • 刊出日期:  2015-06-24

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