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胶质瘤中MicroRNA-184的表达及其预后价值

郎博娟, 马金阳, 胡余昌, 陈路, 唐立华

郎博娟, 马金阳, 胡余昌, 陈路, 唐立华. 胶质瘤中MicroRNA-184的表达及其预后价值[J]. 肿瘤防治研究, 2015, 42(05): 483-487. DOI: 10.3971/j.issn.1000-8578.2015.05.014
引用本文: 郎博娟, 马金阳, 胡余昌, 陈路, 唐立华. 胶质瘤中MicroRNA-184的表达及其预后价值[J]. 肿瘤防治研究, 2015, 42(05): 483-487. DOI: 10.3971/j.issn.1000-8578.2015.05.014
LANG Bojuan, MA Jinyang, HU Yuchang, CHEN Lu, TANG Lihua. MicroRNA-184 Expression in Glioma Tissues and Its Prognosis Value[J]. Cancer Research on Prevention and Treatment, 2015, 42(05): 483-487. DOI: 10.3971/j.issn.1000-8578.2015.05.014
Citation: LANG Bojuan, MA Jinyang, HU Yuchang, CHEN Lu, TANG Lihua. MicroRNA-184 Expression in Glioma Tissues and Its Prognosis Value[J]. Cancer Research on Prevention and Treatment, 2015, 42(05): 483-487. DOI: 10.3971/j.issn.1000-8578.2015.05.014

胶质瘤中MicroRNA-184的表达及其预后价值

详细信息
    作者简介:

    郎博娟(1980-),女,硕士,主治医师,主要从事肿瘤病理学研究

    通讯作者:

    马金阳,E-mail: mjylbj@163.com

  • 中图分类号: R739.41

MicroRNA-184 Expression in Glioma Tissues and Its Prognosis Value

  • 摘要: 目的 研究人脑胶质瘤石蜡包埋组织(formalin-fixed paraffin-embedded, FFPE)中微小RNA-184(microRNA-184, miR-184)的表达及其与临床病理特征之间的关系,探讨其在预测胶质瘤患者预后中的价值。方法 采用微阵列芯片法和RT-PCR检测108例胶质瘤FFPE和32例正常脑组织中的miR-184表达,同时Kaplan-Meier法分析胶质瘤患者总生存期和无病生存期情况。结果 胶质瘤组织中miR-184的表达下调,并用RT-PCR进一步验证;miR-184低表达与WHO分级(P=0.007)、Karnofsky 评分(KPS)(P=0.029)、复发时间(P=0.037)和生存时间(P=0.019)显著相关;Kaplan-Meier 分析结果表明,miR-184低表达与高表达患者总生存期(OS)(P=0.001)和无病生存期(DFS)(P=0.006)差异有统计学意义,miR-184低表达患者预后较差。多因素分析显示胶质瘤中miR-184的差异表达是预测OS[风险比(HR):7.52;95%CI:2.63~21.42;P=0.002]和DFS[HR:11.56;95%CI:5.17~25.93;P<0.001]的独立危险因素。结论 miR-184的表达与胶质瘤患者预后显著相关,表明miR-184可作为预测胶质瘤患者预后的独立标志物。

     

    Abstract: Objective To investigate the relationship between microRNA-184(miR-184) expression and clinicopathologic factors in glioma formalin-fixed paraffin-embedded(FFPE) tissues, and to evaluate the role of miR-184 expression in predicting the prognosis of glioma patients. Methods The expression levels of miR-184 were analyzed in 108 gliomas FFPE and 32 normal brain tissues by microarray and real-time PCR (RT-PCR). Survival analysis by Kaplan-Meier method was performed to assess prognostic significance. Results Down-regulation of miR-184 was detected in most primary glioma tissues, which was confirmed by RT-PCR analysis further. Additionally, down-regulation of miR-184 was significantly associated with WHO grade(P=0.007), Karnofsky performance score(KPS)(P=0.029), time to recurrence(P=0.037) and survival time(P=0.019). Using Kaplan-Meier analysis, a comparison of survival curves of low high expresser of miR-184 revealed a highly significant difference in overall survival(OS)(P=0.001) and disease-free survival(DFS)(P=0.006), which suggested that low expression of miR-184 was associated with a worse prognosis. Multivariate analyses showed that miR-184 expression was the independent risk factors for predicting OS [hazard ratio(HR), 7.52; 95%CI: 2.63-21.42; P=0.002] and DFS[HR, 11.56; 95%CI: 5.17-25.93; P<0.001] of glioma patients. Conclusion miR-184 expression is significantly correlated with the prognosis of glioma patients, suggesting that miR-184 may serve as the independent prognostic marker.

     

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出版历程
  • 收稿日期:  2014-10-12
  • 修回日期:  2015-01-19
  • 刊出日期:  2015-05-24

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