Abstract: Objective To investigate the association between blood 25-hydroxyvitamin(OH) D level and colorectal cancer, and to provide evidence for the prevention strategy and understanding of the etiology of colorectal cancer. Methods MEDLINE, EMBASE and other large databases were searched. The prospectivestudies about the association of 25-(OH) D and colorectal cancer published from 2003 to May 2013 were selected. A comprehensive quantitative analysis was performed by Meta-analysis method with Stata11.0 software. Results Meta-analysis results showed that the pooled RRs of colorectal cancer for the highest versus lowest categories of blood 25-(OH)D levels were 0.79(95%CI:0.65~0.95). Stratifying by geographic region, the pooled RRs of colorectal cancer for the highest versus lowest categories of 25-(OH)D levels for studies conducted in the United States, Europe and Asia were 0.78(95%CI:0.60-1.02),0.77(95%CI:0.56-1.06),0.89(95%CI:0.52-1.50), respectively. Egger's test showed that the publication bias had no statistical significance (P>0.05). Begg's funnel plot was symmetric figure, so there was no significant publication bias. Conclusion Blood 25-(OH)D level is associated with the risk of colorectal cancer. Intake of Vitamin D might be useful for the prevention and treatment of colorectal cancer.