Abstract: MicroRNAs(miRNAs) are a class of cell endogenous expressed small noncoding RNAs, which could regulate gene expression at posttranscriptional level. In recent years, many studies showed that miR-320 expression is downregulated in a series of malignant tumors such as colon caner, breast cancer and cholangiocarcinoma, which indicated miR-320 might play an important role in inhibiting tumor progress by suppressing cell proliferation and invasion. In-depth investigation on target genes and the related signaling pathways regulated by miR-320 could help us to explore the underlying molecular mechanisms in tumorgenesis and progression, as well as providing new targets for clinical treatment. In this article, we review the recent advances on miRNA-320 in tumors.