肝癌膜相关抗原Hab18g为CD147分子的鉴定

Human Hepatoma Membrane—associated Antigen Hab18g Is Identified As a CD147 Molecule

摘要: 目的　从蛋白水平验证肝癌膜相关抗原Hab18g为CD147分子，并探讨单抗Hab18和抗CD147mAb识别抗原表位的异同。方法　间接免疫荧光染色，免疫组化ABC法，斑点杂交及FCM加成试验。结果　抗CD147mAb与Hab18两者识别抗原的阳性反应结果一致，先后加用两种单抗所测的平均荧光强度值高于单独用任一种单抗所测的值。结论　Hab 18g应称为CD147分子，Hab 18和抗CD147两株单抗识别抗原不同表位。

Abstract: Objective To identify the Human hepatoma membrane-associated antigen Hab18g is a CD147 molecule from protein level,and the Hab18 and CD147mAb were used to investigate the epitope of antigen. Methods Indirect immunofluoscent;immunohistochemistry ABC method;dot blot and FCM additive test.Reaults Both CD147mAb and Hab18 recognize the same antigen,the mean intensity fluoresence of two kinds of antibodies used is higher than that of one antibody. Conclusion Human hepatoma membrane-associated antigen Hab18g may name as CD147 molecule,and two kinds of antibodies(Hab18 and CD147mAb)recognize the different epitope of the same antigen.