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肝细胞癌 中Survivin mRNA和Survivin-ΔEx3 mRNA的表达及临床意义

杨 黎, 姬少绯, 朱振宇, 张 毅

杨 黎, 姬少绯, 朱振宇, 张 毅. 肝细胞癌 中Survivin mRNA和Survivin-ΔEx3 mRNA的表达及临床意义[J]. 肿瘤防治研究, 2013, 40(12): 1147-1150. DOI: 10.3971/j.issn.1000-8578.2013.12.009
引用本文: 杨 黎, 姬少绯, 朱振宇, 张 毅. 肝细胞癌 中Survivin mRNA和Survivin-ΔEx3 mRNA的表达及临床意义[J]. 肿瘤防治研究, 2013, 40(12): 1147-1150. DOI: 10.3971/j.issn.1000-8578.2013.12.009
YANG Li, JI Shaofei, ZHU Zhenyu, ZHANG Yi. Expression and Significance of Survivin and Survivin-ΔEx3 mRNA in Heplatocellular Carcinoma[J]. Cancer Research on Prevention and Treatment, 2013, 40(12): 1147-1150. DOI: 10.3971/j.issn.1000-8578.2013.12.009
Citation: YANG Li, JI Shaofei, ZHU Zhenyu, ZHANG Yi. Expression and Significance of Survivin and Survivin-ΔEx3 mRNA in Heplatocellular Carcinoma[J]. Cancer Research on Prevention and Treatment, 2013, 40(12): 1147-1150. DOI: 10.3971/j.issn.1000-8578.2013.12.009

肝细胞癌 中Survivin mRNA和Survivin-ΔEx3 mRNA的表达及临床意义

基金项目: 河南省医学科技攻关计划项目(201303025);河南省教育厅科学技术研究重点资助项目(13B320366)
详细信息
    作者简介:

    杨黎(1981-),女,博士,主治医师,主要从事肿瘤分子生物学的研究

  • 中图分类号: R735.7

Expression and Significance of Survivin and Survivin-ΔEx3 mRNA in Heplatocellular Carcinoma

  • 摘要: 目的 探讨凋亡抑制基因Survivin异构体Survivin-ΔEx3在肝细胞癌(Hepatocellular Carcinoma, HCC)中的表达及其与临床病理之间的关系。方法 采用RT-PCR方法对26例肝细胞癌、24例癌旁组织Survivin及Survivin-ΔEx3的mRNA进行检测。分析Survivin-ΔEx3 mRNA表达与临床各项指标(性别、癌组织大小、临床病理分期、TNM分期等)之间的关系,分析Survivin-ΔEx3与野生型肝细胞癌之间的相关性。结果 在26例HCC标本中,Survivin-ΔEx3 mRNA阳性表达15例,占58%,阴性表达11例,占42%。HCC组织的Survivin-ΔEx3 mRNA表达与患者性别、HBsAg、肝硬化、病灶单发或多发瘤、肿瘤的大小、包膜、癌栓及淋巴结转移无显著相关性(P﹥0.05),而与病理学分级和TNM分期有显著相关性(P﹤0.05)。癌旁组织中未见Survivin mRNA表达。Survivin-ΔEx3的表达与Survivin之间无相关性(P﹥0.05)。 结论 Survivin-ΔEx3与HCC的侵袭性和预后明显相关,且Survivin-ΔEx3的表达与Survivin之间无明显关系,可以作为判断HCC侵袭性和进展的一个潜在预测指标。

     

    Abstract: Objective To evaluate the expression and significance of survivin isomer (survivin-ΔEx3) in hepatocellular carcinoma (HCC). Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of survivin and survivin-ΔEx3 in 26 cases of hepatocellular carcinoma, 24 cases of pericarcinomatous liver tissue. The relationship between the expression of survivin-ΔEx3 mRNA and clinicopathological data was determined. The relationship between survivin-ΔEx3 and survivin was analyzed. Results Positive survivin-ΔEx3 mRNA expression was found in 15 out of the 26 (58%) patients but was completely negative in adjacent normal tissues. The expression of survivin-ΔEx3 mRNA in HCC was not correlated with gender, HBsAg, cirrhosis, tumor number, tumor size, tumor encapsulation, tumor thrombus and lymph node metastasis (P>0.05), but signifi cantly correlated with histological differentiation and TNM stage (P<0.05). There was no signifi cant correlation between the expression of survivin-ΔEx3 and survivin (P>0.05). Conclusion The high-expression of survivin-ΔEx3 might evaluate the invasion and progression of HCC, which had no correlation with the expression of survivin.

     

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出版历程
  • 收稿日期:  2012-09-16
  • 修回日期:  2013-02-04
  • 刊出日期:  2013-12-24

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