高级搜索

诱骗受体3在胰腺癌组织中的表达及意义

周 健, 宋世铎, 朱东明, 赵 华, 李德春, 张子祥

周 健, 宋世铎, 朱东明, 赵 华, 李德春, 张子祥. 诱骗受体3在胰腺癌组织中的表达及意义[J]. 肿瘤防治研究, 2013, 40(12): 1143-1146. DOI: 10.3971/j.issn.1000-8578.2013.12.008
引用本文: 周 健, 宋世铎, 朱东明, 赵 华, 李德春, 张子祥. 诱骗受体3在胰腺癌组织中的表达及意义[J]. 肿瘤防治研究, 2013, 40(12): 1143-1146. DOI: 10.3971/j.issn.1000-8578.2013.12.008
ZHOU Jian, SONG Shiduo, ZHU Dongming, ZHAO Hua, LI Dechun, ZHANG Zixiang. Clinical Signifi cance of Decoy Receptor 3 Expression in Pancreatic Carcinoma[J]. Cancer Research on Prevention and Treatment, 2013, 40(12): 1143-1146. DOI: 10.3971/j.issn.1000-8578.2013.12.008
Citation: ZHOU Jian, SONG Shiduo, ZHU Dongming, ZHAO Hua, LI Dechun, ZHANG Zixiang. Clinical Signifi cance of Decoy Receptor 3 Expression in Pancreatic Carcinoma[J]. Cancer Research on Prevention and Treatment, 2013, 40(12): 1143-1146. DOI: 10.3971/j.issn.1000-8578.2013.12.008

诱骗受体3在胰腺癌组织中的表达及意义

基金项目: 江苏省普通高校研究生科研创新计划资助项目(CXLX11_0088);苏州市科教兴卫资助项目(8WKQ0802)
详细信息
    作者简介:

    周健(1976-),男,博士在读,主治医师,主要从事胰腺癌基础和临床研究

    通讯作者:

    张子祥,E-mail:zj0612@163.com

  • 中图分类号: R735.9

Clinical Signifi cance of Decoy Receptor 3 Expression in Pancreatic Carcinoma

  • 摘要: 目的 探讨诱骗受体3(decoy receptor,DcR3)在胰腺癌组织中的表达及其与预后生存的相关性。方法 通过免疫组织化学和ELISA分别检测组织和血清中DcR3蛋白的表达,并分析其与预后生存的关系。结果 DcR3阳性细胞染色定位于胞质中,胰腺癌组织中DcR3阳性率为50 %(25/50),而非癌胰腺组织中DcR3阳性率为24%(12/50),两者差异有统计学意义(P<0.05)。术前胰腺癌患者血清中DcR3为(73.71±18.26)pg/ml,与胰腺囊腺瘤组(7.96±1.25)pg/ml相比,差异有统计学意义(P<0.01)。胰腺癌组术后DcR3水平(19.76±4.52)pg/ml明显下降,与术前相比,有统计学差异(P<0.01)。50例随访患者中位生存时间为27.4月。DcR3在血清中的表达水平与患者术后总体生存时间有关 (P<0.05)。Cox回归分析显示与胰腺癌患者总体生存时间相关的指标有肿瘤大小、TNM分期、淋巴转移和血清中DcR3的表达水平。结论 (1)胰腺癌组织和血清中DcR3蛋白高表达;(2)胰腺癌患者血清中DcR3的水平将有助于肿瘤的临床诊断和预后判断。

     

    Abstract: Objective To investigate the expression of DcR3 and correlation between DcR3 and prognosis in pancreatic carcinoma. Methods DcR3 protein expressions in tissues and sera were respectively detected by i mmunohistochemistry(IHC) and ELISA. Correlations between DcR3 expression and prognosis were analyzed. Results DcR3 staining positive cells were located in cytoplasm. The positive rate of DcR3 in pancreatic carcinoma tissues was 50 % (25/50) higher than that in non-cancerous tissues was (24%, 12/50), (P < 0.05). The level of DcR3 in serum of pancreatic carcinoma before operation was (73.71±18.26)pg/ml, showing a signifi cant difference compared to cystadenoma(7.96±1.25) pg/ml (P<0.01),and signifi cantly decreased after operation (19.76±4.52) pg/ml, P < 0.01.The median survival time of these patients was 27.4 months. Patients with higher levels of DcR3 had signifi cantly shorter survival time than those with lower levels of DcR3(P <0.05). The cox regression analysis showed tumor size, TNM stage, lymph node metastasis, and levels of DcR3 were associated with survival. Conclusions DcR3 protein was over-expressed in tissues and sera of pancreatic carcinoma. DcR3 in serum should be considered as a novel parameter for clinical diagnosis and prognostic judgement in pancreatic carcinoma.

     

  • [1] Li W, Zhang C, Chen C, et al. Correlation between expression of DcR3 on tumor cells and sensitivity to FasL[J]. Cell Mol Immunol,2007,4(6): 455-60.
    [2] Bamias G, Siakavellas SI, Stamatelopoulos KS, et al. Circulating levels of TNF-like cytokine 1A (TL1A) and its decoy receptor 3 (DcR3) in rheumatoid arthritis[J].Clin Immunol, 2008, 12 9(2):249-55.
    [3] Gill RM, Hunt JS. Soluble receptor (DcR3) and cellular inhibitor of apoptosis-2 (cIAP-2) protect human cytotrophoblast cells against LIGHT-mediated apoptosis[J]. Am J Pathol,2004, 165(1): 30 9-17.
    [4] Koichi O, Seiji H, Midori S, et al. Amplifi cation and expression of a decoy receptor for Fas ligand (DcR3) in virus (EBV or HTLV-I) associated lymphomas[J]. Cancer Lett,2000, 160(1): 89-97.
    [5] Chen L, Tian X, Li W, et al. Expressions of Fas/DcR3 and RGDFasL mediated apoptosis in pituitary adenomas[J]. Neurol India,2009, 57(1): 28-30 .
    [6] Mueller AM, Pedre X, Killian S, et al. The Decoy Receptor 3 (DcR3, TNFRSF6B) suppresses Th17 immune responses and is abundant in human cerebrospinal fl uid[J]. J Neuroimmunol,2009, 20 9(1-2): 57-64.
    [7] Macher-Goeppinger S, Aulmann S, Wagener N, et al. Decoy receptor 3 is a prognostic factor in renal cell cancer[J]. Neoplasia, 20 08,10(10):1049-56.
    [8] Pitti RM, Marsters SA, Lawrence DA, et al. Genomic amplifi cation of a decoy receptor for Fas ligand in lung and colon cancer[J]. Nature,1998, 396(6712):699-703.
    [9] Tsuji S, Hosotani R, Yonehara S, et al. Endogenous decoy receptor 3 blocks the growth inhibition signals mediated by Fas ligand in human pancreatic adenocarcinoma[J]. Int J Cancer,2003, 10 6(1):17-25.
    [10] Morishige T, Yoshioka Y, Inakura H, et al. Creation of a LIGHT mutant with the capacity to evade the decoy receptor for cancer therapy[J]. Biomaterials,2010, 31(12):3357-63.
    [11] Yu KY, Kwon B, Ni J, et al. A newly identifi ed member of tumor necrosis factor receptor superfamily (TR6) suppresses LIGHTmediated apoptosis[J]. J Biol Chem,1999, 274(20):13733-6.
    [12] You RI, Chang YC, Chen PM, et al. Apoptosis of dendritic cell induced by decoy receptor 3(DcR3) [J]. Blood, 2008, 11 1(3):1480-8.
    [13] Yang CR, Hsieh SL, Teng CM, et al. Soluble decoy receptor 3 induces angiogenesis by neutralization of TL1A, a cytokine belonging to tumor necrosis factor superfamily and exhibiting angiostatic action[J]. Cancer Res,2004,64(3):1122-9.
    [14] Wu Y, Guo E, Yu J, et al. High DcR3 expression predicts stage pN2-3 in gastric cancer[J]. Am J Clin Oncol,2008,31(1):79-83.
    [15] Liang QL, Wang BR, Li GH. DcR3 and survivin are highly expressed in colorectal carcinoma and closely correlated to its clinicopathologic parameters[J]. J Zhejiang Univ Sci B,2009,10(9):675-82.
    [16] Li H, Zhang L, Lou H, et al. Overexpression of decoy receptor 3 in precancerous lesions and adenocarcinoma of the esophagus[J]. Am J Clin Pathol,2005,124(2):282-7.
    [17] Chen C,Zhang C,Zhuang G, et al. Decoy receptor 3 overexpression and immunologic tolerance in hepatocellular carcinoma (HCC) development[J]. Cancer Invest,??2008, 26(10):965-74.
    [18] Arakawa Y, Tachibana O, Hasegawa M, et al. Frequent gene amplification and overexpression of decoy receptor 3 in glioblastoma[J]. Acta Neuropathol,2005,109(3):294-8.
    [19] Connor JP, Felder M. Ascites from epithelial ovarian cancer contain high levels of functional decoy receptor 3 (DcR3) and is associated with platinum resistance[J]. Gynecol Oncol, 20 08,111(2):330-5.
    [20] Hsu TL, Chang YC, Chen SJ, et al. Modulation of dendritic cell differentiation and maturation by decoy receptor 3[J]. J Immunol, 20 02,168(10):4846-53.
    [21] Hsu TL, Wu YY, Chang YC, et al. Attenuation of Th1 response in decoy receptor 3 transgenic mice[J]. J Immunol,2005,175(8):5135 -45.
    [22] Wu Y, Han B, Sheng H, et al. Clinical signifi cance of detecting elevated serum DcR3/TR6/M68 in malignant tumor patients[J]. Int J Cancer,2003,105(5):724-32.
计量
  • 文章访问数:  1168
  • HTML全文浏览量:  29
  • PDF下载量:  429
  • 被引次数: 0
出版历程
  • 收稿日期:  2012-09-02
  • 修回日期:  2012-10-19
  • 刊出日期:  2013-12-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭