高级搜索

宫颈癌组织中OPNHIF-1α的表达及其与新辅助化疗敏感度的关系

王君霞, 李 阳, 关 婷

王君霞, 李 阳, 关 婷. 宫颈癌组织中OPNHIF-1α的表达及其与新辅助化疗敏感度的关系[J]. 肿瘤防治研究, 2013, 40(11): 1064-1069. DOI: 10.3971/j.issn.1000-8578.2013.11.012
引用本文: 王君霞, 李 阳, 关 婷. 宫颈癌组织中OPNHIF-1α的表达及其与新辅助化疗敏感度的关系[J]. 肿瘤防治研究, 2013, 40(11): 1064-1069. DOI: 10.3971/j.issn.1000-8578.2013.11.012
WANG Junxia, LI Yang, GUAN Ting. Relationship between Expressions of OPN and HIF-1α and Neoadjuvant Chemotherapy Sensitivity in Cervical Carcinoma Tissues[J]. Cancer Research on Prevention and Treatment, 2013, 40(11): 1064-1069. DOI: 10.3971/j.issn.1000-8578.2013.11.012
Citation: WANG Junxia, LI Yang, GUAN Ting. Relationship between Expressions of OPN and HIF-1α and Neoadjuvant Chemotherapy Sensitivity in Cervical Carcinoma Tissues[J]. Cancer Research on Prevention and Treatment, 2013, 40(11): 1064-1069. DOI: 10.3971/j.issn.1000-8578.2013.11.012

宫颈癌组织中OPNHIF-1α的表达及其与新辅助化疗敏感度的关系

基金项目: 广东省科技计划资助项目(2011B031800203)
详细信息
    作者简介:

    王君霞(1984-),女,硕士,住院医师,主要从事妇科肿瘤的研究

    通讯作者:

    关婷,E-mail:lxgdut@163.com

  • 中图分类号: R737.33

Relationship between Expressions of OPN and HIF-1α and Neoadjuvant Chemotherapy Sensitivity in Cervical Carcinoma Tissues

  • 摘要: 目的 探讨新辅助化疗前、后宫颈癌组织OPN mRNA与HIF-1α mRNA的表达变化,分析其作为宫颈癌新辅助化疗敏感度预测因子的可能性。方法 采用实时荧光定量PCR法检测20例宫颈癌患者新辅助化疗前、后组织中OPN mRNA与HIF-1α mRNA的表达水平。结果 宫颈癌患者新辅助化疗后组织中OPN mRNA与HIF-1α mRNA的表达水平明显下降(P<0.05)。新辅助化疗有效组化疗前、后组织中OPN mRNA与HIF-1α mRNA表达水平均明显低于无效组(P=0.016,P=0.001)。结论 宫颈癌患者组织中OPN mRNA与HIF-1α mRNA的表达水平与化疗的敏感性及临床疗效关系密切,可作为宫颈癌新辅助化疗敏感度的重要预测因子。

     

    Abstract: Objective To investigate the mRNA expression and significance of OPN and HIF-1α pre- and post-neoadjuvant chemotherapy in cervical carcinoma, and analyze its possibility to be the predictors for neoadjuvant chemotherapy sensitivity. Methods Real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR) method was used to detect the mRNA levels of OPN and HIF-1α in pre- and postneoadjuvant chemotherapy in 20 cases of cervical carcinoma tissues. Results The mRNA levels of OPN and HIF-1α were signifi cantly decreased after neoadjuvant chemotherapy in patients with cervical cancer tissues (P<0.05). mRNA levels of OPN and HIF-1α in effective chemotherapy group were lower than ineffective group pre- and post-neoadjuvant chemotherapy(P=0.016, P=0.001). Conclusion OPN and HIF-1α mRNA levels were signifi cantly associated with neoadjuvant chemotherapy chemosensitivity and they might be used as predictors for neoadjuvant chemotherapy sensitivity in cervical carcinoma.

     

  • [1] Song SQ,Zhang GN.Current assist treatment of cervical carcinoma[J].Shi Yong Yi Xue Lin Chuang Za Zhi, 20 05,2(2):22-5. [宋水勤,张国楠.宫颈癌新辅助化疗现状[J].实 用医学临床杂志,2005,2(2):22-5.]
    [2] Standal T,Boret M,Sundan A.Role of osteopontin in adhension,migration,cell survival and bone remodeling[J].Exp Oncol,2004,26(3):179-84.
    [3] Li Y,Li L,Wang JT,et al.Elevated content of osteopontin in plasma and tumor tissues of patients with laryngeal and hypopharyngeal carcinoma associated with metastasis and prognosis[J]. Med Oncol,2012,29(3):1429-34.
    [4] Chen RX,Xia YH,Cui JF,et al. Osteopontin,a single marker for predicting the prognosis of patients with tumor-node-metastasis stage I hepatocellular carcinoma after surgical resection[J].J Gastroenterol Hepatol, 2010,25(8):1435-42.
    [5] Snitcovsky I,Leitao GM,Pasini FS,et al.Plasma osteopontin levels in patients with head and neck cancer undergoing chemoradiotherapy[J]. Arch Otolaryngol Head Neck Surg, 20 09,135(8):807-11.
    [6] Fukuba H,Yamashita H,Nagano Y,et al.Siah-1 facilitates ubiquitination and degradation of factor inhibiting HIF-1 alpha (HIF-1a) [J]. Biochem Biophys Res Commun,2007,353(2):324-9.
    [7] Berta MA,Mazure N,Hattab M, et al.Sumoylation of hypoxiainducible factor-1 alpha reduces its transcriptional activity[J]. Biochem Biophys Res Commun,2007,360(3):646-52.
    [8] Semenza GL. Hypoxia-inducible factors: mediators of cancer progression and targets for cancer therapy[J].Trends Pharmacol Sci,2012,33(4):207-14.
    [9] Yang G,Zhang Y,Wu J,et al.Osteopontin regulates growth and migration of human nasopharyngeal cancer cells[J].Mol Med Rep,2011,4(6):1169-73.
    [10] Luczak MW,Roszak A,Pawlik P,et al.Increased expression of HIF-1A and its implication in the hypoxia pathway in primary advanced uterine cervical carcinoma[J].Oncol Rep,2011,26(5):1259-64.
    [11] Liu J,Zhang J,Wang X,et al.HIF-1 and NDRG2 contribute to hypoxia-induced radioresistance of cervical cancer Hela cells[J]. Exp Cell Res,2010,316(12):1985-93.
    [12] Geng J,Tang J,Zhao Y, et al.An evaluation of application value of three-dimensional power Doppler sonography in cervical cancer and cervical intraepithelialneoplasia grade III(CIN 3)[J]. Zhongguo Fu Chan Ke Lin Chuang Za Zhi,2011,12(1):22-5.[耿 京, 唐军, 赵旸, 等. 探讨三维能量多普勒超声在宫颈癌与 重度宫颈上皮内瘤变的应用价值[ J ] .中国妇产科临床杂 志,2011,12(1):22-5.]
计量
  • 文章访问数:  1161
  • HTML全文浏览量:  36
  • PDF下载量:  571
  • 被引次数: 0
出版历程
  • 收稿日期:  2012-09-02
  • 修回日期:  2012-12-04
  • 刊出日期:  2013-11-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭