高级搜索

基膜聚糖基因在侵袭转移子宫内膜癌组织中的表达

张慧峰, 熊世禄, 李岽健, 杨 镇

张慧峰, 熊世禄, 李岽健, 杨 镇. 基膜聚糖基因在侵袭转移子宫内膜癌组织中的表达[J]. 肿瘤防治研究, 2013, 40(08): 789-792. DOI: 10.3971/j.issn.1000-8578.2013.08.015
引用本文: 张慧峰, 熊世禄, 李岽健, 杨 镇. 基膜聚糖基因在侵袭转移子宫内膜癌组织中的表达[J]. 肿瘤防治研究, 2013, 40(08): 789-792. DOI: 10.3971/j.issn.1000-8578.2013.08.015
ZHANG Huifeng, XIONG Shilu, LI DongJian, YANG Zhen. Expression of Lumican in Invasive and Metastatic Endometrial Carcinoma Tissues[J]. Cancer Research on Prevention and Treatment, 2013, 40(08): 789-792. DOI: 10.3971/j.issn.1000-8578.2013.08.015
Citation: ZHANG Huifeng, XIONG Shilu, LI DongJian, YANG Zhen. Expression of Lumican in Invasive and Metastatic Endometrial Carcinoma Tissues[J]. Cancer Research on Prevention and Treatment, 2013, 40(08): 789-792. DOI: 10.3971/j.issn.1000-8578.2013.08.015

基膜聚糖基因在侵袭转移子宫内膜癌组织中的表达

基金项目: 国家自然科学基金资助项目(30901430)
详细信息
    作者简介:

    张慧峰(1977-),男,硕士,主治医师,主要从事妇科恶性肿瘤的发病机制与诊疗的研究

  • 中图分类号: R737.33

Expression of Lumican in Invasive and Metastatic Endometrial Carcinoma Tissues

  • 摘要: 目的 探讨不同侵袭、转移性的子宫内膜癌组织中基膜聚糖基因(Lumican)的表达差异性。 方法 选取48例子宫内膜癌以及正常内膜组织,采用半定量RT-PCR及Western blot分别检测Lumican mRNA及蛋白表达水平,SPSS13.0软件包统计分析Lumican在不同侵袭、转移性的子宫内膜癌组织中的表达差异性。 结果 Lumican mRNA及蛋白在内膜癌组织中表达明显低于正常内膜组织[(0.54±0.042)vs. (0.87±0.035);(0.42±0.026)vs. (0.83±0.037)],且表达水平间的差异有统计学意义(P<0.05)。48例子宫内膜癌组织中,子宫浅肌层侵犯组(22例)、子宫深肌层侵犯组(11例)或宫颈及宫旁侵犯组(11例)、淋巴结转移组(4例)四组之间组织中Lumican mRNA及蛋白表达差异有统计学意义(P<0.05);子宫深肌层侵犯与宫颈及宫旁侵犯组之间Lumican表达差异无统计学意义(P>0.05)。 结论 Lumican在子宫内膜癌组织中表达水平下调;Lumican的表达与子宫内膜癌组织侵袭、转移性负相关。Lumican的低表达可能参与子宫内膜癌的侵袭、转移恶性生物学行为过程。

     

    Abstract: Objective To explore the expression profiles of Lumican in invasive and metastatic endometrial carcinoma. Methods Forty-eight cases of endometrial carcinoma and paired normal endometrium tissues were applied for detecting the expression of Lumican. The mRNA and protein expression profiles of Lumican were analysed by RT-PCR and Western blot, respectively. SPSS13.0 statistical software was adopted to analyze the expression difference of Lumican in endometrial carcinoma. Results Compared with the normal endometrium tissues, mRNA and protein expression of Lumican were down-regulated in endometrial carcinoma tissues [(0.54±0.042)vs. (0.87±0.035);(0.42±0.026) vs. (0.83±0.037)], respectively (P<0.05). It was showed evidently difference of Lumican expression in 48 cases of endometrial carcinoma, including superficial invasive group (22 cases), deep invasive group (11 cases) /cervix invasive group (11 cases) and lymph metastatic group (4 cases). Conclusion Lumican was downregulated in endometrial carcinoma, Lumican expression was negatively related to the invasion and metastasis of endometrial carcinoma. Low expression of Lumican may be involved in the malignant biological behavior of invasion and metastasis in the endometrial carcinoma.

     

  • [1] Blochberger TC, Cornuet PK, Hassell JR. Isolation and partial characterization of lumican and decorin from adult chicken corneas. A keratan sulfate-containing isoform of decorin is developmentally regulated[J].J Biol Chem,1992,267(29):20613-9.
    [2] Blochberger TC, Vergnes JP, Hempel J, et al. cDNA to chick lumican (corneal keratan sulfate proteoglycan) reveals homology to the small interstitial proteoglycan gene family and expression in muscle and intestine[J]. J Biol Chem,1992,267(1):347-52.
    [3] Chakravarti S, Magnuson T, Lass JH, et al. Lumican regulates collagen fibril assembly: skin fragility and corneal opacity in the absence of lumican[J]. J Cell Biol,1998,141(5):1227-86.
    [4] Funderburegh JL, Funderburgh ML, Mann MM, et al. Arterial lunican. Properties of a corneal-type keratan sulfate proteoglycan from bovine aorta[J]. J Biol Chem,1991,266(36):24773-7.
    [5] Ping Lu Y, Ishiwata T, Asano G. Lumican expression in alpha cells of islets in pancreas and pancreatic cancer cells[J]. J Pathol,2002,196(3):324-30.
    [6] Chakravarti S, Magnuson T, Lass JH, et al. Lumican regulates collagen fibril assembly: skin fragility and corneal opacity in the absence of lumican[J]. J Cell Biol,1998,141(5):1277-86.
    [7] Chakravarti S, Peitroll WM, Hassell JR, et al. Corneal opacity in lumican-null mice: defects in collagen fibril structure and packing in the posterior stroma[J]. Invest Ophthalmol Vis Sci,2000,41(11):3365-73.
    [8] Jepsen KJ, Wu F, Perngallo JH, et al. A syndrome of joint laxity and impaired tendon integrity in lumican- and fibromodulin-deficient mice[J].J Biol Chem,2002,277(38):35532-40.
    [9] Chakravarti S, Paul J, Roberts L, et al. Ocular and scleral alterations in gene-targeted lumican-fibromodulin double-null mice[J]. Invest Ophthalmol Vis Sci,2003,44(6):2422-32.
    [10] Ishiwata T, Fujii T, Ishiwata S, et al. Effect of morpholino antisense oligonucleotide against lumican mRNA in human enbryonic kidney (HEK) 293 cells[J]. Pathol Int,2004,54(2):77-81.
    [11] Vuillermoz B, Khoruzhenko A, D'Onofrio MF, et al. The small leucine-rich proteoglycan lumican inhibits melanoma progression[J]. Exp Cell Res,2004,296(2):294-306.
    [12] Nikitovic D, Berdiaki A, Zafiropoulos A, et al. Lumican expression is positively correlated with the differentiation and negatively with the growth of human osteosarcoma cells[J]. FEBS J,2008,275(2):350-61.
    [13] D'Onofrio MF, Br zlllon S, Baranck T, et al. Identification of beta1 integrin as mediator of melanoma cell adhesion to lumican[J]. Biochem Biophys Res Commun,2008,365(2):266-72.
    [14] Leygue E, Snell L, Dotzlaw H, et al. Expression of lumican in human breast carcinoma[J]. Cancer Res,1998,58(7):1348-52.
    [15] Troup S, Njue C, Klfewer EV, et al. Reduced expression of the small leucine-rich proteoglycans lumican and decorin is associated with poor outcome in node-negative invasive breast cancer[J]. Clin Cancer Res,2003,9(1):207-14.
    [16] Lu YP, Ishiwata T, Kawahara K, et al. Expression of lumican in human colorectal cancer cells[J]. Pathol Int,2002,52(8):519-26.
    [17] Leygue E, Snell L, Dotzlaw H, et al. Lumican and decorin are differentially expressed in human breast carcinoma[J]. J Pathol,2000,192(3):313-20.
    [18] Luo HR, Liu LX, Jiang HC. Expression of lumican mRNA in gastric cancer and its clinical significance[J].Zhongguo Pu Wai Ji Chu Yu Lin Chuang Za Zhi, 2007,14(5):551-3.[罗海蓉,刘连新,姜洪池.抑癌基因Lumican在胃癌中的表达及其临床意义[J].中国普外基础与临床杂志, 2007, 14(5):551-3.]
    [19] Yoshioka N, Inoue H, Nakanlshi K, et al. Isolation of transformation suppressor genes by cDNA subtraction: lumican suppresses transformation induced by v-src and v-K-ras[J]. J Virol,2000,74(2):1008-13.
    [20] Yamaguchi Y, Mann DM, Ruoslutti E. Negative regulation of transforming growth factor-beta by the proteoglycan decorin[J]. Nature,1990,346(6281):281-4.
计量
  • 文章访问数:  1626
  • HTML全文浏览量:  16
  • PDF下载量:  414
  • 被引次数: 0
出版历程
  • 收稿日期:  2012-09-03
  • 修回日期:  2013-01-15
  • 刊出日期:  2013-08-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭