Abstract: Objective To study the heterogcneous immbilized intact cells as potential cancer vaccines and its anti-tumor immune response mechanism. Methods Mice were immunized with immobilized human lung cancer cells and inoculated spontaneous lung adenocarcinoma of mouse to observe effect of tumor cellular immunity on growth of mouse cancer. Infiltrated T cells in tumor tissues and effect of immune serum on growth of human lung cancer cells were detected by immunohistochemistry and MTT. Results Immunizing mice with intact immobilized human lung adenocarcinoma and squamous carcinoma can inhibit growth of mice lung adenocarcinoma (P＜0.05) with inhibit rate of 40.8% and 49.2% respectively，which has significantly advantage than the effect of cell debris from human lung adenocarcinoma and squamous carcinoma. Immune serum of mice can inhibit growth of human lung cancer cells in vitro. Conclusion Heterologous human immobilized intact lung cancer cells induce effective humoral immune response against mice lung cancer of mice and inhibit growth of tumor obviously，the inhibitory effect is maybe related to induced functional anti-cancer neutralizing antibody in mice.