Clinicopathologic Significance of LIMK1 Expression in Human Colon Cancer
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摘要: 目的 研究LIMK1表达与结肠癌发生、分化程度、肿瘤大小、淋巴结转移、临床分期的关系。 方法 将87例结肠癌、26例腺瘤及34例正常结肠组织共147例结肠标本制成组织芯片，采用免疫组织化学技术检测结肠癌、腺瘤及正常组织中LIMK1表达。 结果 LIMK1在腺癌组织中表达[75.86% (66/87)]明显高于在腺瘤[38.46% (10/26)]与结肠正常黏膜[20.58%(7/34)]组织中的表达（P＜0.05），而腺瘤组织中的表达高于正常黏膜组织（P＜0.05）。高分化结肠癌组织中LIMK1表达率[40.00% (4/10)]明显高于中分化结肠癌组织中的表达率[70.21%(33/47)]与低分化腺癌结肠癌组织中的表达率[96.67% (29/30)]（P＜0.05），而中分化结肠癌组织中的表达率显著高于低分化腺癌（P＜0.05）。体积＜5.0 cm结肠癌组织LIMK1表达[57.14%(20/35)]明显低于≥5.0 cm的表达[88.46%(46/52)]（P＜0.05）。淋巴结转移表达97.78%(44/45)显著高于无淋巴结转移的表达[52.38% (22/42)]（P＜0.05）。Dukes分期A+B组LIMK1表达[ 60.98%（25/41）]明显低于C+D组[89.13%（41/46）]（P＜0.05）。 结论 LIMK1表达与结肠癌的发生、分化程度、肿瘤大小、淋巴结转移、临床分期密切相关，其可能是结肠癌侵袭转移的重要标志。Abstract: Objective To investigate the relations between LIMK1 expression and carcinogenesis, tumor size, pathological classification, lymph node metastasis and clinical stages in human colon cancer. Methods Immunohistochemistry method using tissue chip was used to examine the expression of LIMK1 in colon cancer (87 cases), adenoma (26 cases) and normal tissue (34 cases). Results The expression level of LIMK1 in colon cancer was 75.86% (66/87),and significantly higher than 20.58% (7/34) in normal tissue and 38.46% (10/26) in adenoma, respectively.LIMK1 in adenoma was significantly higher than that in the normal tissue (P＜0.05). LIMK1 expression in well-differentiated was 40.00% (4/10) and lower than 70.21% (33/47) in moderately differentiated and 96.67% (29/30) in poorly differentiated adenocarcinoma, LIMK1 expression in moderately differentiated was lower than that in poorly differentiated adenocarcinoma (P＜0.05). The expression of LIMK1 in volume＜5.0 cm of colon cancer was lower than that in volume ≥5.0 cm,57.14%(20/35) vs.88.46% (46/52) (P＜0.05). The expression of LIMK1 of lymph node metastasis was increased compared with non-lymph nale metastasis 97.78% (44/45) vs.52.38% (22/42) (P＜0.05). The expression of LIMK1 in Dukes stage A and B was significantly lower than in C and D 60.98% (25/41) vs.89.13% (41/46) (P＜0.05). There was no significantly correlation between age and the expression of LIMK1 (P＞0.05). Conclusion The expression of LIMK1 is closely related to carcinogenesis, tumor size, pathological classification, lymph node metastasis and clinical stage in colon cancer, suggesting that LIMK1 may be a important biomarker of invasion and metastasis.
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Key words:
- LIMK1 /
- Colon cancer /
- Tissue chip /
- Immunohisochemistry
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