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热疗联合人肿瘤坏死因子对TNFR1高表达胶质瘤的细胞周期、F-肌动蛋白及其侵袭性的影响

秦丽娟, 张军伟, 张田, 王艳蕾, 张一兵, 周洪霞, 贾永森, 王树华

秦丽娟, 张军伟, 张田, 王艳蕾, 张一兵, 周洪霞, 贾永森, 王树华. 热疗联合人肿瘤坏死因子对TNFR1高表达胶质瘤的细胞周期、F-肌动蛋白及其侵袭性的影响[J]. 肿瘤防治研究, 2013, 40(06): 551-554. DOI: 10.3971/j.issn.1000-8578.2013.06.011
引用本文: 秦丽娟, 张军伟, 张田, 王艳蕾, 张一兵, 周洪霞, 贾永森, 王树华. 热疗联合人肿瘤坏死因子对TNFR1高表达胶质瘤的细胞周期、F-肌动蛋白及其侵袭性的影响[J]. 肿瘤防治研究, 2013, 40(06): 551-554. DOI: 10.3971/j.issn.1000-8578.2013.06.011
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QIN Lijuan, ZHANG Junwei, ZHANG Tian, WANG Yanlei, ZHANG Yibing, ZHOU Hongxia, JIA Yongsen, WANG Shuhua. Effect of Hyperthermia Combined with rhTNF on Cell Cycle,F-actin and Invasiveness to Over-expressed TNFR1 Glioma Cells[J]. Cancer Research on Prevention and Treatment, 2013, 40(06): 551-554. DOI: 10.3971/j.issn.1000-8578.2013.06.011
Citation: QIN Lijuan, ZHANG Junwei, ZHANG Tian, WANG Yanlei, ZHANG Yibing, ZHOU Hongxia, JIA Yongsen, WANG Shuhua. Effect of Hyperthermia Combined with rhTNF on Cell Cycle,F-actin and Invasiveness to Over-expressed TNFR1 Glioma Cells[J]. Cancer Research on Prevention and Treatment, 2013, 40(06): 551-554. DOI: 10.3971/j.issn.1000-8578.2013.06.011
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热疗联合人肿瘤坏死因子对TNFR1高表达胶质瘤的细胞周期、F-肌动蛋白及其侵袭性的影响

  • 1.063000 河北唐山，河北联合大学基础医学院；2.河北联合大学附属医院；3.河北联合大学中医学院

基金项目: 河北省卫生厅科学研究基金项目(20120144)
详细信息
    作者简介:

    秦丽娟（1975-）,女，博士，副教授，主要从事脑肿瘤的相关研究

  • 中图分类号: R739.41

Effect of Hyperthermia Combined with rhTNF on Cell Cycle,F-actin and Invasiveness to Over-expressed TNFR1 Glioma Cells

  • 1.College of Basic Medical Sciences,Hebei United University,Tangshan 063000,China; 2.Hebie United University Affiliated Hospital;3.College of Traditional Chinese Medicine,Hebei United University

摘要

    摘要
  • 摘要: 目的 探讨热疗联合重组人肿瘤坏死因子（recombinant human tumor necrosis factor，rhTNF）对肿瘤坏死因子受体1 (tumor necrosis factor receptor 1,TNFR1)高表达的胶质瘤细胞的细胞周期和F-肌动蛋白(F-actin)的影响及其与胶质瘤侵袭性的关系。 方法 建立TNFR1高表达胶质瘤细胞株，RT-PCR和Western blot法检测胶质瘤细胞TNFR1的表达水平；碘化丙啶染色后用流式细胞术检测胶质瘤细胞细胞周期的变化；WST-8法检测细胞增殖；免疫荧光技术检测胶质瘤细胞内F-actin的表达水平；Transwell小室法检测胶质瘤细胞侵袭性改变。 结果 与对照组相比，TNFR1高表达胶质瘤细胞株的TNFR1 mRNA水平增加了78.5%，其蛋白质的表达水平增加了89.7% (P＜0.05)；经热疗联合rhTNF处理后细胞增殖受抑制，S和G2/M期的TNFR1高表达胶质瘤细胞数之和明显增多，而F-actin的荧光强度和胶质瘤侵袭性分别降低了72.3% 和83.10%。 结论 热疗联合rhTNF可能是通过阻滞TNFR1高表达胶质瘤细胞的细胞周期和降低F-actin的表达来实现降低胶质瘤侵袭性的作用。

     

    Abstract: Objective The study objective was to investigate the effect of hyperthermia combined with rhTNF on cell cycle and F-actin of TNFR1 in over-expressed glioma, as well as invasiveness in vitro. Methods C6 cell Line of over-expressed TNFR1 (C6/TNFR1) was constructed., The mRNA and protein of TNFR1 were measured by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot respectively, and the cell cycle and cell proliferation were determined by flow cytometry(stained by propidium iodide) and WST-8 respectively. The invasiveness was measured by transwell assay and immunofluorescence technique was used to measure F-actin protein expression. Results Compared with the control group,the mRNA and protein levels of TNFR1 in c6/TNFR1 cell was increased, by 78.5% and 89.7% (P<0.05), respectively.The cell proliferation was inhibited and most of c6/TNFR1 cells were arrested in S+G2/M phase compared with the control group cells after hyperthermia combined with rhTNF treatment (P<0.05).The fluorescence intensity of F-actin and the average number of C6/TNFR1 cells passing through the inserted filter were decreased by 72.3% and 83.10% respectively, compared to the control group cells after hyperthermia combined with rhTNF treatment (P<0.01).  Conclusion Hyperthermia combined with rhTNF might reduce glioma of C6/TNFR1 invasiveness through blocking cell cycle and reducing the expression of F-action.

     

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出版历程
  • 收稿日期:  2012-08-01
  • 修回日期:  2012-12-16
  • 刊出日期:  2013-06-24

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