高级搜索

microRNA-93在胃癌中的表达及其临床意义

张广钰, 田小林, 钟 漓, 戴 凌, 朱袭嘉, 蒋志庆

张广钰, 田小林, 钟 漓, 戴 凌, 朱袭嘉, 蒋志庆. microRNA-93在胃癌中的表达及其临床意义[J]. 肿瘤防治研究, 2013, 40(05): 447-450. DOI: 10.3971/j.issn.1000-8578.2013.05.009
引用本文: 张广钰, 田小林, 钟 漓, 戴 凌, 朱袭嘉, 蒋志庆. microRNA-93在胃癌中的表达及其临床意义[J]. 肿瘤防治研究, 2013, 40(05): 447-450. DOI: 10.3971/j.issn.1000-8578.2013.05.009
ZHANG Guangyu, TIAN Xiaolin, ZHONG Li, DAI Ling, ZHU Xijia, JIANG Zhiqing. Clinical Significance of microRNA-93 Expression in Gastric Tumor[J]. Cancer Research on Prevention and Treatment, 2013, 40(05): 447-450. DOI: 10.3971/j.issn.1000-8578.2013.05.009
Citation: ZHANG Guangyu, TIAN Xiaolin, ZHONG Li, DAI Ling, ZHU Xijia, JIANG Zhiqing. Clinical Significance of microRNA-93 Expression in Gastric Tumor[J]. Cancer Research on Prevention and Treatment, 2013, 40(05): 447-450. DOI: 10.3971/j.issn.1000-8578.2013.05.009

microRNA-93在胃癌中的表达及其临床意义

详细信息
    作者简介:

    张广钰(1966-),男,硕士,副主任医师,主要从事消化道肿瘤的临床与基础研究

    通讯作者:

    钟漓,E-mail:acrosssky@126.com

  • 中图分类号: R735.2

Clinical Significance of microRNA-93 Expression in Gastric Tumor

  • 摘要: 目的 探讨microRNA-93在胃癌中的表达情况及其与临床病例特征的相关性。方法 提取60例胃癌及其相应正常胃黏膜组织标本的总RNA,采用RT-qPCR方法检测microRNA-93的相对表达量,并分析其与临床TNM分期、淋巴结转移、分化程度以及肿瘤治疗疗效和预后等临床病理因素的关系。结果 胃癌组织与癌旁正常组织比较,microRNA-93在胃癌组织中表达上调,而且其表达与TNM分期和淋巴结转移有显著相关性(P<0.05)。以相对表达量≥2.0为高表达为界限,进一步的多因素分析发现TNM分期、淋巴结转移、microRNA-93的表达与患者的预后显著相关(P<0.01)。microRNA-93的高表达降低了患者的疗效。结论 microRNA-93 在胃癌组织中高表达,尤其是晚期转移性胃癌,提示microRNA-93在胃癌中是一种重要的癌基因。microRNA-93可能作为胃癌诊断和预后判断的分子标志物。

     

    Abstract: Objective To investigate the expression of microRNA-93 and analyze their correlation with clinic-pathological features in gastric tumor. Methods The expression levels of microRNA-93 in 60 gastric tumors and their matched non-tumor adjacent tissue specimens were detected using stem-loop real-time reverse transcription-polymerase chain reaction (RT-qPCR), and their correlation with clinic-pathologic features,cancer therapeutics and prognosis of gastric tumor was analyzed. Results Expression levels of microRNA-93 were significantly higher in tumor tissues than that in adjacent normal tissues (P<0.05). The expression of microRNA-93 was associated with TNM stage (P<0.05) and lymphatic metastasis (P<0.05). The high expression of microRNA-93 group was the comparative expression more than 2.0. Multivariate analysis showed the prognosis was statistically correlated with TNM stage,lymphatic metastasis and microRNA-93 expression(P<0.01).The high expression of microRNA-93 reduced the effect of cancer therapeutics. Conclusion microRNA-93 is highly elevated in gastric cancer, especially in advanced and metastasized gastric cancer, suggesting microRNA-93 plays critical roles in carcinogenesis of gastric cancer. microRNA-93 might play an important role in gastric tumor and function as the potential gene therapy target for this tumor.

     

  • [1] Sempere LF. Integrating contextual miRNA and protein signatures for diagnostic and treatment decisions in cancer[J]. Expert Rev Mol Diagn,2011,11(8):813-27.
    [2] Wang F, Fu XD, Zhou Y, et al. Down-regulation of the cyclin E1 oncogene expression by microRNA-16-1 induces cell cycle arrest in human cancer cells[J]. BMB Rep,2009,42(11):725-30.
    [3] Imam JS, Buddavarapu K, Lee-Chang JS, et al. MicroRNA-185 suppresses tumor growth and progression by targeting the Six1 oncogene in human cancers[J]. Oncogene,2010,29(35):4971-9.
    [4] Petrocca F, Visone R, Onelli M R, et al. E2F1-regulated microRNAs impair TGFbeta-dependent cell-cycle arrest and apoptosis in gastric cancer[J].Cancer Cell,2008,13(3):272-86.
    [5] Chen Y, Gorski DH. Regulation of angiogenesis through a microRNA (miR-130a) that down- regulates antiangiogenic homeobox genes GAX and HOXA5[J]. Blood,2008,111(3):1217-26.
    [6] Chen Y, Zhu X, Zhang X, et al. Nanoparticles modified with tumor-targeting scFv deliver siRNA and miRNA for cancer therapy[J]. Mol Ther,2010,18(9):1650-6.
    [7] Cerne JZ, Gersak K, Novakovic S. The influence of the genetic variant within miRNA-binding site in estrogen receptor alpha gene on the risk of breast cancer in postmenopausal women on hormone replacement therapy[J]. Cancer Biomark,2010-2011,8(3):123-8.
    [8] He L, Thomson JM, Hemann MT, et al. A microRNA polycistron as a potential human oncogene[J] . Nature,2005,435(7043):828-33.
    [9] Huang V, Place RF, Portnoy V, et al. Upregulation of Cyclin B1 by miRNA and its implications in cancer[J]. Nucleic Acids Res,2012,40(4):1695-707.
    [10] Nishida N, Nagahara M, Sato T, et al. Microarray analysis of colorectal cancer stromal tissue reveals upregulation of two oncogenic miRNA clusters[J]. Clin Cancer Res,2012,18(11):3054-70.
    [11] Fang L, Deng Z, Shatseva T, et al. MicroRNA miR-93 promotes tumor growth and angiogenesis by targeting integrin-beta8[J]. Oncogene,2011,30(7):806-21.
    [12] Fu X, Tian J, Zhang L, et al. Involvement of microRNA-93, a new regulator of PTEN/Akt signaling pathway, in regulation of chemotherapeutic drug cisplatin chemosensitivity in ovarian cancer  cells[J]. FEBS Lett,2012,586(9):1279-86.
    [13] Montanini L, Lasagna L, Barili V, et al. MicroRNA cloning and sequencing in osteosarcoma cell lines: differential role of  miR-93[J]. Cell Oncol (Dordr),2012,35(1):29-41.
    [14] Song J, Bai Z, Han W, et al. Identification of suitable reference genes for qPCR analysis of serum microRNA in gastric cancer patients[J]. Dig Dis Sci,2012,57(4):897-904
计量
  • 文章访问数:  1841
  • HTML全文浏览量:  17
  • PDF下载量:  352
  • 被引次数: 0
出版历程
  • 收稿日期:  2012-06-03
  • 修回日期:  2012-09-13
  • 刊出日期:  2013-05-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭