Abstract: Objective To investigate the relationship between HER-2, anemia, prealbumin and the prognosis of gastric cancer. Methods One hundred and three patients diagnosed as primary gastric cancer and staged at Ⅲ-Ⅳ from 2008 to 2009 were retrospectively analyzed. The clinical characteristics such as age, gender, histological type, tumor location, tumor size, infiltration depth, lymph node metastasis, clinical stage, the expression of HER-2, anemia and prealbumin concentration were observed. Survival curves were drawn with the Kaplan-Meier analysis. Comparisons were made with the Log rank test. Cox proportion risk model was established and χ²-test or Fisher's exact probability test was used to compare clinical characteristics. Results Lymph node metastasis, clinical stage, the expression of HER-2, anemia and prealbumin were identified as influencing factors about the prognosis of gastric cancer, while age, gender, histological type, tumor location and size were irrelevant with the survival of tumor patients. Multiple logistic regression analysis showed that independent and significant factors associated with the prognosis of gastric cancer were clinical stage, the expression level of HER-2, anemia and prealbumin concentration(P<0.05). Conclusion （1）HER-2 over-expression, anemia and low prealbumin concentration are the independent and significant factors on the prognosis of gastric cancer. （2）Lymph node metastasis is the influencing but not the independent factor on the prognosis of gastric cancer.（3）There is a significant difference on the proportion of anemia between HER-2 positive group and negative group, and the same result is found in stage Ⅲ group and stage Ⅳ group but not in prealbumin groups.