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SHG-44与U251胶质瘤细胞株放射抵抗性差异及其与APEX1 mRNA表达和细胞周期分布的关系

张兴逵, 杨智勇, 邓兴力, 刘永贵, 杨德标

张兴逵, 杨智勇, 邓兴力, 刘永贵, 杨德标. SHG-44与U251胶质瘤细胞株放射抵抗性差异及其与APEX1 mRNA表达和细胞周期分布的关系[J]. 肿瘤防治研究, 2013, 40(05): 425-429. DOI: 10.3971/j.issn.1000-8578.2013.05.004
引用本文: 张兴逵, 杨智勇, 邓兴力, 刘永贵, 杨德标. SHG-44与U251胶质瘤细胞株放射抵抗性差异及其与APEX1 mRNA表达和细胞周期分布的关系[J]. 肿瘤防治研究, 2013, 40(05): 425-429. DOI: 10.3971/j.issn.1000-8578.2013.05.004
ZHANG Xingkui, YANG Zhiyong, DENG Xingli, LIU Yonggui, YANG Debiao. Radioresistance Difference between SHG-44 and U251 Glioma Cell Lines and Relationship among Radioresistance, APEX1 mRNA Expression and Cell Cycle Distribution[J]. Cancer Research on Prevention and Treatment, 2013, 40(05): 425-429. DOI: 10.3971/j.issn.1000-8578.2013.05.004
Citation: ZHANG Xingkui, YANG Zhiyong, DENG Xingli, LIU Yonggui, YANG Debiao. Radioresistance Difference between SHG-44 and U251 Glioma Cell Lines and Relationship among Radioresistance, APEX1 mRNA Expression and Cell Cycle Distribution[J]. Cancer Research on Prevention and Treatment, 2013, 40(05): 425-429. DOI: 10.3971/j.issn.1000-8578.2013.05.004

SHG-44与U251胶质瘤细胞株放射抵抗性差异及其与APEX1 mRNA表达和细胞周期分布的关系

详细信息
    作者简介:

    张兴逵(1984-),男,硕士在读,主要从事神经肿瘤的基础及临床治疗

    通讯作者:

    杨智勇,E-mail:13808721500@163.com

  • 中图分类号: R739.41

Radioresistance Difference between SHG-44 and U251 Glioma Cell Lines and Relationship among Radioresistance, APEX1 mRNA Expression and Cell Cycle Distribution

  • 摘要: 目的 对不同级别的SHG-44与U251胶质瘤细胞株放射抵抗性差异进行比较,探索放射抵抗性与APEX1 mRNA表达及细胞周期分布的关系。 方法 采用平板克隆形成实验法检测SHG-44细胞株与U251细胞株之间放射抵抗性的差别;采用RT-PCR技术检测已知的胶质瘤放射抵抗因子APEX1 mRNA 的表达情况;流式细胞术检测两种细胞细胞周期的分布情况;直线相关分析分析细胞放射抵抗性与APEX1 mRNA的表达情况及细胞周期分布的关系。 结果 与WHOⅣ级的U251相比,WHOⅡ~Ⅲ级的SHG-44的放射抵抗性较高(SF2U251=0.58±0.02,SF2SHG-44=0.70±0.15,t=3.19,P<0.05),但其APEX1 mRNA表达要低(1.17±0.04 vs. 0.70±0.18,t=19.92,P<0.05),G1期SHG-44比例高于U251(60.13±3.26 vs.51.72±5.14,t=2.51,P<0.05),S期SHG-44低于U251(18.57±0.64 vs. 28.80±2.96,t=5.09,P<0.05),G2期两者差异无统计学意义(17.63±3.91 vs. 21.78±4.81,t=1.25,P>0.05),G1期比例与SF2存在相关性(r=0.735,P<0.05)。 结论 胶质瘤的放射抵抗性与病理级别可能呈负相关,不同类型胶质瘤的放射抵抗机制可能存在差异,APEX1并不是SHG-44细胞放射抵抗性增高的决定因素,G1期阻滞可能是SHG-44细胞放射抵抗性增高的原因之一。

     

    Abstract: Objective To explore the radioresistance difference between SHG-44 and U251 cell line,investigate the correlation among radioresistance, APEX1 mRNA expression and cell cycle distribution. Methods Colony formation assay was performed to determine the radioresistance difference, APEX1 mRNA expression was determined by RT-PCR,cell cycle distribution was investigated using flow cytometry, their correlation were analyzed by linear correlation. Results Compared with U251(pathological grade: WHO Ⅳ),SHG-44(pathological grade: WHO Ⅱ-Ⅲ) was higher radioresistant(SF2U251=0.58±0.02,SF2SHG-44=0.70±0.15,t=3.19,P<0.05),but its APEX1 mRNA expression was lower(1.17±0.04 vs.0.70±0.18,t=19.92,P<0.05),its G1 phase proportion was increased(60.13±3.26 vs. 51.72±5.14,t=2.51,P<0.05),S phase proportion was decreased(18.57±0.64vs. 28.80±2.96,t=5.09,P<0.05),and there is no significant difference in G2 phase between U251 and SHG-44(17.63±3.91 vs. 21.78±4.81,t=1.25,P>0.05),G1 phase arrest was correlated to radioresistance(r=0.735,P<0.05). Conclusion Pathological grade and radioresistance may be negatively correlated in glioma,there may be different radioresistance mechanisms in different types of gliomas, APEX1 is not correlated with radioresistance in glioma SHG-44,but G1 phase may be correlated with radioresistance in glioma SHG-44.

     

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出版历程
  • 收稿日期:  2012-08-01
  • 修回日期:  2012-11-15
  • 刊出日期:  2013-05-24

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