Effect of 5-Aza-CdR on Invasion and Migration of Pancreatic Carcinoma Cell Line PANC-1
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摘要: 目的 研究甲基化抑制物5-氮杂-2-脱氧胞苷(5-Aza-CdR)干预对胰腺癌细胞株PANC-1侵袭和迁移能力的影响，初步探讨其发挥作用的机制。方法 用不同浓度的5-Aza-CdR处理PANC-1细胞株,采用划痕迁移试验和Transwell小室法观察干预前后细胞迁移和侵袭能力的改变，免疫荧光染色法检测干预前后细胞MMP-2的表达。结果 经5-Aza-CdR干预后，与对照组相比，PANC-1细胞的迁移和侵袭能力明显减弱（P<0.05），细胞内MMP-2的表达降低(P<0.05)。结论 5-Aza-CdR可以明显抑制胰腺癌细胞株PANC-1的侵袭转移能力，其机制可能是通过抑制MMP-2的表达而降低肿瘤细胞的侵袭和转移。
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关键词:
- 胰腺癌 /
- 5-氮杂-2-脱氧胞苷 /
- MMP-2
Abstract: Objective To investigate the effects of methylation inhibitor 5-Aza-2-deoxycyt(5-Aza-CdR) on the invasion and migration of pancreatic carcinoma cell line PANC-1,and to explore its possible mechanisms. Methods PANC-1 cell line was treated by 5-Aza-CdR of different concentrations.Wound scratch assay and transwell chamber were used to detect the ability of the invation and migration of PANC-1 cells.The expression of MMP-2 was detected by immunofluorescent staining. Results After being treated by 5-Aza-CdR,compared with the control group, the ability of migration and invasion was reduced obviously(P<0.05). Immunocytochemical analysis indicated that the expression of MMP-2 was down-regulated after being treated by 5-Aza-CdR (P<0.05). Conclusion 5-Aza-CdR can inhibit the invasive ability of PANC-1 cells, and its mechanism might be reducing the invasion and migration of the cells by inhibiting the expression of MMP-2.-
Key words:
- Pancreatic carcinoma /
- 5-Aza-2-deoxycyt /
- MMP-2
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