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5-氮杂-2-脱氧胞苷对人胰腺癌细胞株PANC-1侵袭和迁移能力的影响

王蒙, 汤志刚, 黄强, 任维华

王蒙, 汤志刚, 黄强, 任维华. 5-氮杂-2-脱氧胞苷对人胰腺癌细胞株PANC-1侵袭和迁移能力的影响[J]. 肿瘤防治研究, 2013, 40(04): 332-335. DOI: 10.3971/j.issn.1000-8578.2013.04.004
引用本文: 王蒙, 汤志刚, 黄强, 任维华. 5-氮杂-2-脱氧胞苷对人胰腺癌细胞株PANC-1侵袭和迁移能力的影响[J]. 肿瘤防治研究, 2013, 40(04): 332-335. DOI: 10.3971/j.issn.1000-8578.2013.04.004
WANG Meng, TANG Zhigang, HUANG Qiang, REN Weihua. Effect of 5-Aza-CdR on Invasion and Migration of Pancreatic Carcinoma Cell Line PANC-1[J]. Cancer Research on Prevention and Treatment, 2013, 40(04): 332-335. DOI: 10.3971/j.issn.1000-8578.2013.04.004
Citation: WANG Meng, TANG Zhigang, HUANG Qiang, REN Weihua. Effect of 5-Aza-CdR on Invasion and Migration of Pancreatic Carcinoma Cell Line PANC-1[J]. Cancer Research on Prevention and Treatment, 2013, 40(04): 332-335. DOI: 10.3971/j.issn.1000-8578.2013.04.004

5-氮杂-2-脱氧胞苷对人胰腺癌细胞株PANC-1侵袭和迁移能力的影响

基金项目: 国家自然科学基金资助项目(81071734)
详细信息
    作者简介:

    王蒙(1987-),男,硕士在读,主要从事胰腺肿瘤的研究

    通讯作者:

    汤志刚,E-mail:tzg7031@163.com

  • 中图分类号: R735.9

Effect of 5-Aza-CdR on Invasion and Migration of Pancreatic Carcinoma Cell Line PANC-1

  • 摘要: 目的 研究甲基化抑制物5-氮杂-2-脱氧胞苷(5-Aza-CdR)干预对胰腺癌细胞株PANC-1侵袭和迁移能力的影响,初步探讨其发挥作用的机制。方法 用不同浓度的5-Aza-CdR处理PANC-1细胞株,采用划痕迁移试验和Transwell小室法观察干预前后细胞迁移和侵袭能力的改变,免疫荧光染色法检测干预前后细胞MMP-2的表达。结果 经5-Aza-CdR干预后,与对照组相比,PANC-1细胞的迁移和侵袭能力明显减弱(P<0.05),细胞内MMP-2的表达降低(P<0.05)。结论 5-Aza-CdR可以明显抑制胰腺癌细胞株PANC-1的侵袭转移能力,其机制可能是通过抑制MMP-2的表达而降低肿瘤细胞的侵袭和转移。

     

    Abstract: Objective To investigate the effects of methylation inhibitor 5-Aza-2-deoxycyt(5-Aza-CdR) on the invasion and migration of pancreatic carcinoma cell line PANC-1,and to explore its possible mechanisms. Methods PANC-1 cell line was treated by 5-Aza-CdR of different concentrations.Wound scratch assay and transwell chamber were used to detect the ability of the invation and migration of PANC-1 cells.The expression of MMP-2 was detected by immunofluorescent staining. Results After being treated by 5-Aza-CdR,compared with the control group, the ability of migration and invasion was reduced obviously(P<0.05). Immunocytochemical analysis indicated that the expression of MMP-2 was down-regulated after being treated by 5-Aza-CdR (P<0.05). Conclusion 5-Aza-CdR can inhibit the invasive ability of PANC-1 cells, and its mechanism might be reducing the invasion and migration of the cells by inhibiting the expression of MMP-2.

     

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出版历程
  • 收稿日期:  2012-06-20
  • 修回日期:  2012-09-09
  • 刊出日期:  2013-04-24

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