高级搜索

Notch2在喉鳞状细胞癌中的表达及意义

丁永军, 张建斌, 李曼, 陈晨, 韩继波, 陶泽璋, 陈始明

丁永军, 张建斌, 李曼, 陈晨, 韩继波, 陶泽璋, 陈始明. Notch2在喉鳞状细胞癌中的表达及意义[J]. 肿瘤防治研究, 2013, 40(02): 180-184. DOI: 10.3971/j.issn.1000-8578.2013.02.014
引用本文: 丁永军, 张建斌, 李曼, 陈晨, 韩继波, 陶泽璋, 陈始明. Notch2在喉鳞状细胞癌中的表达及意义[J]. 肿瘤防治研究, 2013, 40(02): 180-184. DOI: 10.3971/j.issn.1000-8578.2013.02.014
Ding Yongjun, Zhang Jianbin, Li Man, Chen Chen, Han Jibo, Tao Zezhang, Chen Shiming. Expressions and Significance of Notch2 Protein in Laryngeal Squamous Cell Carcinoma[J]. Cancer Research on Prevention and Treatment, 2013, 40(02): 180-184. DOI: 10.3971/j.issn.1000-8578.2013.02.014
Citation: Ding Yongjun, Zhang Jianbin, Li Man, Chen Chen, Han Jibo, Tao Zezhang, Chen Shiming. Expressions and Significance of Notch2 Protein in Laryngeal Squamous Cell Carcinoma[J]. Cancer Research on Prevention and Treatment, 2013, 40(02): 180-184. DOI: 10.3971/j.issn.1000-8578.2013.02.014

Notch2在喉鳞状细胞癌中的表达及意义

基金项目: 国家自然科学基金资助项目(81172569)
详细信息
    作者简介:

    丁永军(1985-),男,硕士在读,住院医师,主要从事头颈部肿瘤的基础和临床研究

    通讯作者:

    陈始明,E-mail:shimingchen@163.com

  • 中图分类号: R737.9

Expressions and Significance of Notch2 Protein in Laryngeal Squamous Cell Carcinoma

  • 摘要: 目的探讨Notch2在喉癌组织及体外培养的喉鳞癌细胞Hep-2中的表达及与喉癌患者临床病理资料之间的关系。方法应用量子点超敏免疫荧光染色法检测95例喉癌组织标本中Notch2蛋白的表达,同时选取31例声带息肉标本作为对照组,结合患者临床病理资料进行分析;制作细胞爬片并进行量子点(QDs)免疫荧光染色检测Notch2蛋白在喉鳞癌细胞Hep-2中的表达。结果95例喉癌组织中Notch2阳性表达率为92.6%(88/95),其阳性率与声带息肉中的阳性率(32.3%)相比差异均有统计学意义(P<0.01),其表达主要定位于细胞核。Notch2在喉癌组织中的表达与临床分期显著相关(P<0.05):临床分期越高,表达率越高。Notch2在喉癌组织细胞核中表达阳性与T分级、临床分期、病理分级和淋巴结转移显著相关(P<0.05)。喉癌细胞Hep-2中Notch2表达阳性,其表达主要定位于细胞质和胞膜中。结论Notch2在喉癌的发生发展中起着重要作用,其可能成为治疗喉癌的分子靶点。

     

    Abstract: ObjectiveTo investigate the expressions of Notch2 protein in laryngeal carcinoma cells (Hep-2) and the relationship between the expression and the clinical and pathological data in laryngeal carcinoma. Methods The expressions of Notch2 in 95 cases of laryngeal carcinoma tissue and 31 cases of vocal cord polyps,as well as laryngeal carcinoma cells (Hep-2),were detected by QDs-IgG conjugate immunofluorescence staining,and the relationship of the expressions of Notch2 to clinical and pathological features of laryngeal carcinoma was analyzed. Results The expression positive rate of Notch2-positive in laryngeal carcinoma was 92.6%(88/95),and higher than that in vocal cord polyp(32.3%)(P<0.01).Notch2 expression was mainly localized in the nucleus.Notch2 expression in laryngeal carcinoma was significantly associated with clinical stage (P<0.05).The higher clinical stage had higher expression rate.Notch2 expression in laryngeal carcinoma was significantly related to T classification,clinical stage,histological grade and lymph node metastasis (P<0.05).Laryngeal carcinoma cells Hep-2 had the Notch2 expression and mainly was localized in the cytoplasm and enveloped membrane. Conclusion Notch2 played an important role in development of laryngeal carcinoma,and might be a new molecular target for laryngeal carcinoma.

     

  • [1] Borggrefe T,Oswald F.The Notch signaling pathway:transcriptional regulation at Notch target genes[J].Cell Mol Life Sci,2009,66(10):1631-46.
    [2] Harrison H,Farnie G,Brennan KR,et al.Breast cancer stem cells:something out of notching?[J].Cancer Res,2010,70(22):8973-6.
    [3] Westhoff B,Colaluca IN,D'Ario G,et al.Alterations of the Notch pathway in lung cancer[J].Proc Natl Acad Sci U S A,2009,106(52):22293-8.
    [4] Maliekal TT,Bajaj J,Giri V,et al.The role of Notch signaling in human cervical cancer:implications for solid tumors[J].Oncogene,2008,27(38):5110-4.
    [5] Chen SM,Liu JP,Zhou JX,et al.Suppression of the notch signaling pathway by gamma-secretase inhibitor GSI inhibits human nasopharyngeal carcinoma cell proliferation[J].Cancer Lett,2011,306(1):76-84.
    [6] Gotte M,Greve B,Kelsch R,et al.The adult stem cell marker musashi-1 modulates endometrial carcinoma cell cycle progression and apoptosis via notch-1 and p21(WAF1/CIP1)[J].Int J Cancer,2011,129(8):2042-9.
    [7] Nishina S,Shiraha H,Nakanishi Y,et al.Restored expression of the tumor suppressor gene RUNX3 reduces cancer stem cells in hepatocellular carcinoma by suppressing Jagged1-Notch signaling[J].Oncol Rep,2011,26(3):523-31.
    [8] Jiao J,Qin Z,Li S,et al.Potential role of Notch1 signaling pathway in laryngeal squamous cell carcinoma cell line Hep-2 involving proliferation inhibition,cell cycle arrest,cell apoptosis,and cell migration[J].Oncol Rep,2009,22(4):815-23.
    [9] Xu CZ,Dong P,Wang PH,et al.The expression of Notch1 and its significance in laryngeal carcinoma[J].Zhongguo Er Bi Yan Hou Lu Di Wai Ke Za Zhi,2011,17(6):401-5.[徐成志,董频,王珮华,等.Notch1在喉癌组织中的表达及意义[J].中国耳鼻咽喉颅底外科杂志,2011,17(6):401-5.]
    [10] Cheng F,Yu W,Zhang X,et al.Quantum-dot-based technology for sensitive and stable detection of prostate stem cell antigen expression in human transitional cell carcinoma[J].Int J Biol Markers,2009,24(4):271-6.
    [11] Chu D,Li Y,Wang W,et al.High level of Notch1 protein is associated with poor overall survival in colorectal cancer[J].Ann Surg Oncol,2010,17(5):1337-42.
    [12] Li-Shishido S,Watanabe TM,Tada H,et al.Reduction in nonfluorescence state of quantum dots on an immunofluorescence staining[J].Biochem Biophys Res Commun,2006,351(1):7-13.
    [13] Brocke T,Bootsmann MT,Tews M,et al.Spectroscopy of few-electron collective excitations in charge-tunable artificial atoms[J].Phys Rev Lett,2003,91(25):257401.
    [14] Chen HL,Zhang YX,Xia D,et al.Antigen detection by quantum dots technology in tissue microarray[J].Zhonghua Bing Li Xue Za Zhi,2008,37(6):416-7.[陈洪雷,张玉霞,夏东,等.在组织芯片上利用量子点技术进行抗原的检测[J].中华病理学杂志,2008,37(6):416-7.]
    [15] Moore AE,Sabachewsky L,Toolan HW.Culture characteristics of four permanent lines of human cancer cells[J].Cancer Res,1955,15(9):598-602.
    [16] Doucas H,Mann CD,Sutton CD,et al.Expression of nuclear Notch3 in pancreatic adenocarcinomas is associated with adverse clinical features,and correlates with the expression of STAT3 and phosphorylated Akt[J].J Surg Oncol,2008,97(1):63-8.
    [17] Purow BW,Haque RM,Noel MW,et al.Expression of Notch-1 and its ligands,Delta-like-1 and Jagged-1,is critical for glioma cell survival and proliferation[J].Cancer Res,2005,65(6):2353-63.
    [18] Gao J,Song Z,Chen Y,et al.Deregulated expression of Notch receptors in human hepatocellular carcinoma[J].Dig Liver Dis,2008,40(2):114-21.
    [19] Yeasmin S,Nakayama K,Rahman MT,et al.Expression of nuclear Notch3 in cervical squamous cell carcinomas and its association with adverse clinical outcomes[J].Gynecol Oncol,2010,117(3):409-16.
    [20] Zhang Y,Li B,Ji ZZ,et al.Notch1 regulates the growth of human colon cancers[J].Cancer,2010,116(22):5207-18.
计量
  • 文章访问数:  1500
  • HTML全文浏览量:  20
  • PDF下载量:  504
  • 被引次数: 0
出版历程
  • 收稿日期:  2012-06-17
  • 修回日期:  2012-09-06
  • 刊出日期:  2013-02-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭