二醇组人参皂苷抑制Lewis肺癌生长及NF-κB相关基因的作用
Inhibitory Effects of Ginsenosides on Tumor Growth and NF-κB-related Gene Expression
-
摘要: 目的 研究二醇组人参皂苷Rh2、PPD对Lewis肺癌生长及NF-κB相关信号的抑制作用。方法体外实验采用四甲基偶氮唑盐(MTT)比色法测定Rh2、PPD对Lewis肺癌细胞增殖的影响。体内实验，以足趾皮下接种模型检测Rh2、PPD对Lewis肺癌细胞为瘤源的肿瘤生长的抑制作用。并对体内样品用实时荧光定量PCR方法测定Rh2、PPD对p65和p50(组成性NF-κB)及其下游若干相关基因的表达调节。结果在体外，人参皂苷Rh2、PPD对肺癌细胞株具有明显的增殖抑制作用；在体内，Rh2、PPD对小鼠Lewis肺癌移植性模型均具有明显的生长抑制作用；实时荧光定量PCR结果表明，Lewis肺癌组织在体内经人参皂苷Rh2、PPD作用后，p65和p50及其下游基因表达明显下调。结论Rh2、PPD对Lewis肺癌生长有明显的抑制作用，同时抑制NF-κB及下游基因的表达。Abstract: Objective To investigate the inhibitory effects on growth and NF-κB-related gene expression of Lewis lung cancer cells exposure to ginsenosides Rh2 and PPD in vitro and in vivo. Methods Using MTT assay to determine growth inhibition of Rh2 and PPD in Lewis lung cancer cells.Growth inhibitory efficacies of Rh2 and PPD were determined by subcutaneously implanted Lewis lung cancer cells into C57 mice.Expression of NF-κB (p65 and p50) and relevant down-stream genes were measured using real-time PCR technique. Results Rh2 and PPD significantly reduced cell viability of Lewis lung cancer in vitro.Rh2 and PPD also potently inhibited the growth of lung cancer xenograft in vivo.In the meantime,expressions of p65,p50 and down-stream genes were significantly down-regulated. Conclusion Rh2 and PPD possess strong inhibitory effect on tumor growth while testing with Lewis lung cancer cells in vitro and in vivo.They also down-regulate the expression of NF-κB and its down-stream genes.
下载:
