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上皮性卵巢癌组织中WWOX基因启动子区域CpG岛的甲基化状态及临床意义

闫洪超, 孙洁芸, 陆晓媛, 韩秋峪, 魏敏

闫洪超, 孙洁芸, 陆晓媛, 韩秋峪, 魏敏. 上皮性卵巢癌组织中WWOX基因启动子区域CpG岛的甲基化状态及临床意义[J]. 肿瘤防治研究, 2012, 39(12): 1469-1473. DOI: 10.3971/j.issn.1000-8578.2012.12.015
引用本文: 闫洪超, 孙洁芸, 陆晓媛, 韩秋峪, 魏敏. 上皮性卵巢癌组织中WWOX基因启动子区域CpG岛的甲基化状态及临床意义[J]. 肿瘤防治研究, 2012, 39(12): 1469-1473. DOI: 10.3971/j.issn.1000-8578.2012.12.015
Yan Hongchao, Sun Jieyun, Lu Xiaoyuan, Han Qiuyu, Wei Min. Methylation State of WWOX Gene Promoter CpG Islands in Epithelial Ovarian Cancer and Its Clinical Significance[J]. Cancer Research on Prevention and Treatment, 2012, 39(12): 1469-1473. DOI: 10.3971/j.issn.1000-8578.2012.12.015
Citation: Yan Hongchao, Sun Jieyun, Lu Xiaoyuan, Han Qiuyu, Wei Min. Methylation State of WWOX Gene Promoter CpG Islands in Epithelial Ovarian Cancer and Its Clinical Significance[J]. Cancer Research on Prevention and Treatment, 2012, 39(12): 1469-1473. DOI: 10.3971/j.issn.1000-8578.2012.12.015

上皮性卵巢癌组织中WWOX基因启动子区域CpG岛的甲基化状态及临床意义

基金项目: 徐州市科技发展基金资助项目(XF10C086)
详细信息
    作者简介:

    闫洪超(1973-),男,硕士,副主任医师,主要从事妇科肿瘤的研究

    通讯作者:

    孙洁芸,E-mail:sunjieyun022@sina.com

  • 中图分类号: R737.31

Methylation State of WWOX Gene Promoter CpG Islands in Epithelial Ovarian Cancer and Its Clinical Significance

  • 摘要: 目的 研究上皮性卵巢癌组织中WWOX基因启动子区CpG岛的甲基化状态,并分析WWOX基因的甲基化与上皮性卵巢癌的临床病理指标之间的关系。方法采用甲基化特异性PCR(methylation specific polymerase chain reaction,MSP)方法检测48例上皮性卵巢癌、18例卵巢交界性上皮性肿瘤、26例卵巢良性上皮性肿瘤及33例正常卵巢组织中WWOX基因CpG岛甲基化状态。结果上皮性卵巢癌、卵巢交界性上皮性肿瘤、卵巢良性上皮性肿瘤组织中WWOX基因启动子区CpG岛甲基化率分别为43.75%、26.32%、3.84%,正常卵巢组织中未检测到WWOX基因CpG岛甲基化。上皮性卵巢癌组织中WWOX基因CpG岛的甲基化率明显高于其他卵巢组织,差异有统计学意义(P<0.01)。晚期(Ⅲ期、Ⅳ期)上皮性卵巢癌组织中WWOX基因CpG岛的甲基化率高于早期(Ⅰ期、Ⅱ期)上皮性卵巢癌组织,差异有统计学意义(P<0.05)。结论 上皮性卵巢癌组织中广泛存在着WWOX基因启动子区CpG岛甲基化,可能是导致WWOX基因失活的重要机制。WWOX基因的异常甲基化可能与上皮性卵巢癌的发生发展密切相关,其可能成为上皮性卵巢癌的早期诊断和评估预后的重要指标。

     

    Abstract: Objective To evaluate the methylation status of CpG islands in WWOX gene promoter region of epithelial ovarian cancer,and to explore the relationship between methylation state of WWOX gene CpG island and clinicopathological indexes in epithelial ovarian cancer. Methods The methylation state of WWOX gene CpG island was evaluated by methylation specific polymerase chain reaction(MSP) in 48 patients with epithelial ovarian cancer,18 patients with borderline epithelial ovarian tumors,26 patients with epithelial benign tumors,and 33 patients with normal ovarian tissues. Results The rates of CpG island methylation in WWOX gene promoter region in epithelial ovarian cancer tissues,borderline ovarian tumor tissues and benign ovarian tumor tissues were 43.75%,26.32% and 3.84%,respectively.Normal ovarian tissues were completely unmethylated in WWOX gene CpG island.The rate of CpG island methylation in epithelial ovarian cancer tissues was higher than that in other ovarian tissues,and these differences were found to be statistically significant (P<0.01).The rate of CpG island methylation in WWOX gene promoter region in late-stage (stageⅢ,Ⅳ) epithelial ovarian cancer tissues was higher than that in early-stage (stageⅠ,Ⅱ) epithelial ovarian cancer tissues,and these differences were found to be statistically significant (P<0.05). Conclusion Epithelial ovarian cancer tissues show widespread CpG island methylation in WWOX gene promoter region,which may be explained as the important mechanism that leads to WWOX gene inactivation.A typical methylation of WWOX gene is germane to generation and progression of epithelial ovarian cancer,which makes it probably be an important indicator in epithelial ovarian cancer early diagnosis and prognosis evaluation.

     

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出版历程
  • 收稿日期:  2012-01-12
  • 修回日期:  2012-04-15
  • 刊出日期:  2012-12-24

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