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染色体8q24和17q24区域与中国人列腺癌的关联分析

梁思颖, 王建业, 魏东, 史晓红, 张耀光, 刘铭, 王鑫, 陈鑫, 王飞, 杨帆, 赵帆, 杨泽

梁思颖, 王建业, 魏东, 史晓红, 张耀光, 刘铭, 王鑫, 陈鑫, 王飞, 杨帆, 赵帆, 杨泽. 染色体8q24和17q24区域与中国人列腺癌的关联分析[J]. 肿瘤防治研究, 2012, 39(12): 1442-1446. DOI: 10.3971/j.issn.1000-8578.2012.12.009
引用本文: 梁思颖, 王建业, 魏东, 史晓红, 张耀光, 刘铭, 王鑫, 陈鑫, 王飞, 杨帆, 赵帆, 杨泽. 染色体8q24和17q24区域与中国人列腺癌的关联分析[J]. 肿瘤防治研究, 2012, 39(12): 1442-1446. DOI: 10.3971/j.issn.1000-8578.2012.12.009
Liang Siying, Wang Jiangye, Wei Dong, Shi Xiaohong, Zhang Yaoguang, Liu Ming, Wang Xin, Chen Xin, Wang Fei, Yang Fan, Zhao Fan, Yang Ze. Gene-environment Analysis on Association of Chromosome 8q24 and Chromosome 17q24 with Prostate Cancer in Chinese Population[J]. Cancer Research on Prevention and Treatment, 2012, 39(12): 1442-1446. DOI: 10.3971/j.issn.1000-8578.2012.12.009
Citation: Liang Siying, Wang Jiangye, Wei Dong, Shi Xiaohong, Zhang Yaoguang, Liu Ming, Wang Xin, Chen Xin, Wang Fei, Yang Fan, Zhao Fan, Yang Ze. Gene-environment Analysis on Association of Chromosome 8q24 and Chromosome 17q24 with Prostate Cancer in Chinese Population[J]. Cancer Research on Prevention and Treatment, 2012, 39(12): 1442-1446. DOI: 10.3971/j.issn.1000-8578.2012.12.009

染色体8q24和17q24区域与中国人列腺癌的关联分析

基金项目: 国家自然科学基金资助项目(30972709,81061120527);北京医院重大基金资助项目(BJ-2010-30);卫生部部属医院临床学科重点资助项目(10120101)
详细信息
    作者简介:

    梁思颖(1985-),女,硕士在读,主要从事医学遗传学研究

    通讯作者:

    杨泽,E-mail:yang.ze@live.cn

  • 中图分类号: R394.3;R737.25

Gene-environment Analysis on Association of Chromosome 8q24 and Chromosome 17q24 with Prostate Cancer in Chinese Population

  • 摘要: 目的 探索染色体8q24区域(rs620861C、rs1447295A)和17q24区域(rs185996,G)风险等位基因与北方中国人前列腺癌的关联。了解其基因型与前列腺癌患者表型(年龄、肿瘤分期、PSA水平、Gleason评分、有无良性前列腺肥大)的关联,分析基因之间的交互作用。方法采用病例-对照设计,收集前列腺癌患者临床表型信息;采用PCR-HRM和测序技术对各位点进行了分型,并对基因型在病例组和正常对照组中的分布进行了年龄匹配的病例-对照间比较(病例组:124人,对照组:138人)及基因交互作用分析;对病例组中基因型在不同临床表型中的分布进行了比较。结果在病例-对照之间三个风险位点的基因型和等位基因频率分布差异未见有统计学意义;在病例组中各位点与前列腺癌表型之间未见有显著性关联;未能检测到三个风险位点之间有交互作用。结论染色体8q24区域(rs620861C、rs1447295A)和17q24区域(rs185996G)可能与北方中国人患前列腺癌的遗传风险无关联。

     

    Abstract: Objective To explore whether the risk alleles in Chromosome 8q24(rs620861C,rs1447295A)and Chromosome 17q24(rs1859962G)are associated with prostate cancer in Chinese population;to investigate the association between genotypes and phenotypes(age,T staging of neoplasm,PSA level,Gleason scores and suffering from BPH or not)of PCa patients,to analyse the the interactions among the genes. Methods A case-control study was performed to collect information of PCa patients'clinical features.By using polymerase chain reaction-high resolution melting curve(PCR-HRM)combined with DNA sequencing,each locus was genotyped and examined,and the distribution of each locus' genotype was compared in an age-matched case-control method(124 cases,138 controls),and the gene-gene interaction analysis was done.The contribution of patients′ genotypes in different clinical features was done. Results None of the three variants was observed to show significant difference in the distribution of genotype and gene frequency between cases and controls,neither did they show significant association with PCa phenotypes in cases.There was no significant gene-gene interactions being detected,either. Conclusion Chromosome 8q24(rs620861C,rs1447295A)and Chromosome 17q24(rs1859962G)may have no association with prostate cancer in Chinese population.

     

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出版历程
  • 收稿日期:  2011-11-23
  • 修回日期:  2012-04-27
  • 刊出日期:  2012-12-24

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