高级搜索

CD133和CD44在骨肉瘤中的表达及其临床意义

何爱娜, 祁伟祥, 汤丽娜, 沈赞, 孙元珏, 陈杰, 姚阳

何爱娜, 祁伟祥, 汤丽娜, 沈赞, 孙元珏, 陈杰, 姚阳. CD133和CD44在骨肉瘤中的表达及其临床意义[J]. 肿瘤防治研究, 2012, 39(11): 1328-1332. DOI: 10.3971/j.issn.1000-8578.2012.11.012
引用本文: 何爱娜, 祁伟祥, 汤丽娜, 沈赞, 孙元珏, 陈杰, 姚阳. CD133和CD44在骨肉瘤中的表达及其临床意义[J]. 肿瘤防治研究, 2012, 39(11): 1328-1332. DOI: 10.3971/j.issn.1000-8578.2012.11.012
He Aina, Qi Weixiang, Tang Li′na, Shen Zan, Sun Yuanjue, Chen Jie, Yao Yang. Expression and Clinical Significance of CD133 and CD44 in Osteosarcoma[J]. Cancer Research on Prevention and Treatment, 2012, 39(11): 1328-1332. DOI: 10.3971/j.issn.1000-8578.2012.11.012
Citation: He Aina, Qi Weixiang, Tang Li′na, Shen Zan, Sun Yuanjue, Chen Jie, Yao Yang. Expression and Clinical Significance of CD133 and CD44 in Osteosarcoma[J]. Cancer Research on Prevention and Treatment, 2012, 39(11): 1328-1332. DOI: 10.3971/j.issn.1000-8578.2012.11.012

CD133和CD44在骨肉瘤中的表达及其临床意义

基金项目: 国家自然科学基金资助项目(81001191);上海市科学技术委员会资助课题(10PJ1408300)
详细信息
    作者简介:

    何爱娜(1981-),女,博士,主治医师,主要从事骨肉瘤干细胞的相关研究

    通讯作者:

    姚阳,E-mail:qwxzlk@163.com

  • 中图分类号: R738.1

Expression and Clinical Significance of CD133 and CD44 in Osteosarcoma

  • 摘要: 目的 探讨CD133和CD44在骨肉瘤中的表达情况及其与骨肉瘤患者预后的关系。方法应用免疫组织化学方法检测CD133和CD44在79例骨肉瘤患者石蜡切片标本中的表达情况,应用卡方检验比较CD133、CD44表达与患者临床病理资料的相互关系,Kaplan-Meier法计算患者生存率,Log rank检验比较CD133、CD44阴性低表达组与高表达组患者之间的生存差异,并以性别、年龄、肿瘤部位、肿瘤大小、Ennecking 分期、CD133和CD44表达水平、局部复发及肺转移为变量指标,应用Cox回归模型进行多因素分析,研究这些因素与骨肉瘤患者生存率之间的关系。结果CD133和CD44的表达水平与患者性别、年龄、肿瘤部位、肿瘤大小、Ennecking分期及局部复发无关,发生肺转移的骨肉瘤患者其CD133和CD44的表达明显高于未发生转移的骨肉瘤患者,差异有统计学意义(P值分别为0.00014和0.0008)。经单因素生存分析表明,影响骨肉瘤患者预后的因素有:肿瘤大小,局部复发,肺转移,CD44和CD133表达水平。多因素分析显示CD133和CD44表达水平及肿瘤大小是影响患者预后的独立影响因素。结论CD133和CD44表达水平与骨肉瘤肺转移密切相关,检测CD133和CD44的表达可以作为骨肉瘤患者肺转移及预后预测的指标。

     

    Abstract: Objective To investigate the expressions of CD133 and CD44 in osteosarcoma tissue and their relationship with the prognosis of osteosarcoma. Methods Expressions of CD133 and CD44 were examined by immuno-histochemical MaxVision method in 79 specimens of human osteosarcoma tissues.The relationship between expression of CD133/CD44 and clinicopathological parameters was calculated by Chi-square test.Kaplan-Meier method was used to calculate the overall survival rate.The factors of gender,age,tumor-size,tumor location,Ennecking stage,local recurrence and lung metastasis were included in univariate analysis.The univariate analysis was used to determine the prognostic factors related with survival rate by Log rank test.The COX proportional-hazard regression model was used to analyze the correlation between the prognostic factor and survival. Results No correlations were found between expression of CD133/CD44 and clinicopathological parameters including gender,age,tumor location,tumor size,Ennecking stage and local recurrence,but occurrence of pulmonary metastasis was significantly associated with the expression levels of CD133/CD44(P value was 0.00014 and 0.0008,respectively).Univariate survival analysis showed that the significant prognostic factors were tumor size,local recurrence,lung metastasis,expression levels of CD133 and CD44.Multivariate analysis showed that CD133 and CD44 expression and tumor size were independent prognostic factors of patients with osteosarcoma. Conclusion The positive expressions of CD133 and CD44 closely correlate with the lung metastasis of osteosarcoma,detection of CD133 and CD44 may be used as an indicator for lung metastasis and prognosis of osteosarcoma.

     

  • [1] Raymond AK.Conventional osteosarcoma[M].Lyon:IARC Press,2002:264-70.
    [2] Gibbs CP,Kukekov VG,Reith JD,et al.Stem-like cells in bone sarcomas:implications for tumorigenesis[J].Neoplasia,2005,7(11):967-76.
    [3] Wang L,Park P,Lin CY.Characterization of stem cell attributes in human osteosarcoma cell lines[J].Cancer Biol Ther,2009,8(6):543-52.
    [4] Singh SK,Hawkins C,Clarke ID,et al.Identification of human brain tumour initiating cells[J].Nature,2004,432 (7015):396-401.
    [5] Dalerba P,Dylla SJ,Park IK,et al.Phenotypic characterization of human colorectal cancer stem cells[J].Proc Nail Acad Sci U S A,2007,104(24):10158-63.
    [6] Yin S,Li J,Hu C,et al.CD133 positive hepatocellular carcinoma cells possess high capacity for tumorigenicity[J].Int J Cancer,2007,120(7):1444-50.
    [7] Monzani E,Facchetti F,Galmozzi E,et al.Melanoma contains CD133 and ABCG2 positive cells with enhanced tumorigenic potential[J].Eur J Cancer,2007,43(5):935-46.
    [8] Miraglia S,Godfrey W,Yin AH,et al.A novel five-transmembrane hematopoietic stem cell antigen: isolation,characterization,and molecular cloning[J].Blood,1997,90(12):5013-21.
    [9] Giebel B,Corbeil D,Beckmann J,et al.Segregation of lipid raft markers including CD133 in polarized human hematopoietic stem and progenitor cells[J].Blood,2004,104(8):2332-8.
    [10] Bauer N,Fonseca AV,Florek M,et al.New insights into the cell biology of hematopoietic progenitors by studying prominin-1 (CD133) [J].Cells Tissues Organs,2008,188(1-2):127-38.
    [11] O′Brien CA,Pollett A,Gallinger S,et al.A human colon cancer cell capable of initiating tumour growth in immunodeficient mice[J].Nature,2007,445(7123):106-10.
    [12] Bruno S,Bussolati B,Grange C,et al.CD133+ renal progenitor cells contribute to tumor angiogenesis[J].Am J Pathol,2006,169(6):2223-35.
    [13] Suetsugu A,Nagaki M,Aoki H,et al.Characterization of CD133+ hepatocellular carcinoma cells as cancer stem/progenitor cells[J].Biochem Biophys Res Commun,2006,351(4):820-4.
    [14] Baba T,Convery PA,Matsumura N,et al.Epigenetic regulation of CD133 and tumorigenicity of CD133+ ovarian cancer cells[J].Oncogene,2009,28(2):209-18.
    [15] Tirino V,Desiderio V,d′Aquino R,et al.Detection and characterization of CD133+ cancer stem cells in human solid tumours[J].PLoS One,2008,3(10):e3469.
    [16] Naor D,Sionov RV,Ish-Shalom D.CD44:structure,function,and association with the malignant process [J].Adv Cancer Res,1997,71:241-319.
    [17] Boldrini E,Peres SV,Morini S,et al.Immunoexpression of Ezrin and CD44 in patients with osteosarcoma[J].J Pediatr Hematol Oncol,2010,32(6):e213-7.
计量
  • 文章访问数:  2340
  • HTML全文浏览量:  22
  • PDF下载量:  597
  • 被引次数: 0
出版历程
  • 收稿日期:  2011-12-27
  • 修回日期:  2012-05-02
  • 刊出日期:  2012-11-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭