番茄红素诱导人结肠癌SW480细胞凋亡的作用机制
Mechanism of Lycopene Induced Cell Apoptosis on Human Colon Cancer SW480 Cells
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摘要: 目的 探讨番茄红素诱导人结肠癌SW480细胞凋亡的分子机制。方法体外培养SW480细胞,采用MTT比色实验、流式细胞术、RT-PCR和Western blot方法,分析番茄红素对SW480细胞的生长增殖作用、对细胞凋亡率的影响及对细胞凋亡相关分子Bcl-2、Bax、Caspase-9和Caspase-3表达的影响。结果MTT法显示,番茄红素能显著抑制SW480细胞的生长增殖能力,且呈一定的量效与时效关系,24 h的半数抑制浓度(IC50)为1.0 μmol/L；流式细胞仪分析表明,番茄红素呈浓度依赖性诱导SW480细胞凋亡；RT-PCR或Western blot分析表明,SW480细胞经不同浓度番茄红素诱导后,与溶媒组相比,Bcl-2表达逐渐减弱,而Bax,Caspase-9和Caspase-3表达逐渐增强,Bcl-2/Bax比值降低。结论番茄红素对SW480细胞的抑制增殖作用,可能与降低Bcl-2/Bax比值、增加Caspase-9和Caspase-3蛋白表达、诱导细胞凋亡有关。Abstract: Objective To investigate the effect of Lycopene on the apoptosis of human colon cancer SW480 cells and the relevant molecular mechanism. Methods The anti-proliferative effect of a different concentration of Lycopene on SW480 cells was measured by MTT assay.Ratio of SW480 cells apoptosis was analyzed by flow cytometry.The levels of Bcl-2,Bax were detected by RT-PCR and Western blot.The expression levels of Caspase-9 and Caspase-3 were analyzed by Western blot. Results The MTT assay showed that Lycopene had potent anti-proliferative effect on SW480 cells in a time- and dose-dependent manner.The IC50 value of Lycopene was SW480 cells treated with 1.0 μmol/L Lycopene for 24 hours.Flow cytometry analysis revealed that Lycopene increased the percentage of apoptotic SW480 cells.The expression level of Bcl-2 were decreased,while the expression levels of Bax,Caspase-9 and Caspase-3 were increased significantly in SW480 cells treated with different concentrations of Lycopene. Conclusion Lycopene could induce apoptosis of SW480 cells by decreasing the ratio of Bcl-2/Bax and increasing Caspase-9 and Caspase-3 expression.
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