Annexin 2基因对多发性骨髓瘤细胞侵袭力的影响及相关机制
Influnce and Therapy of Annexin2 in Multiple Myeloma′s Invasion
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摘要: 目的 了解Annexin 2(AnxA2)基因对骨髓瘤U266、RPMI8226细胞侵袭力的影响及相关机制。方法采用siRNA转染人骨髓瘤U266、RPMI8226细胞,应用Real-time PCR、Western blot鉴定干扰效果。继而应用Transwell板检测细胞侵袭力,应用Real-time PCR检测对侵袭相关基因的影响。结果AnxA2 siRNA转染U266和RPMI8226细胞可明显降低细胞侵袭力(P<0.05),同时降低侵袭相关因子MMP-2、MMP-9、MT1-MMP和TIMP-2的表达(P<0.05)。结论AnxA2基因在骨髓瘤细胞U266和RPMI8226侵袭中起促进作用。Abstract: To investigate the influence and therapy of Annexin2(AnxA2) in multiple myeloma′s invasion. Methods After transfection U266 and RPMI8226 cells with the small interfering RNA(siRNA) that targets human AnxA2,the effect of interfering was tested with Real-time PCR and Western blot.Then detected the cell invasion with transwell plates and the influence to the invasive potential factors with real-time PCR. Results Silencing AnxA2 gene by siRNA resulted in the decreased invasive potential of U266 and RPMI8226 cell lines.It could also inhibit the expression of invasive potential factors including MMP-2,MMP-9,MT1-MMP and TIMP-2 in these two cell lines. Conclusion AnxA2 plays a critical role in the invasive potential of MM cell lines U266 and RPMI8226.
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