白花丹素对骨肉瘤细胞系U2OS的作用及其机制
Effect and Mechanism of Plumbagin in Human Osteosarcoma Cell Line U2OS
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摘要: 目的 研究白花丹素对骨肉瘤细胞系U2OS的作用,并初步探讨其作用机制。方法用CCK-8法检测白花丹素对骨肉瘤细胞的促凋亡作用；用HOECHST 33342染色研究白花丹素对骨肉瘤细胞作用后的形态学变化；用Western blot法检测白花丹素作用后骨肉瘤细胞MDM2和p53蛋白表达情况。结果CCK-8结果显示白花丹素对骨肉瘤细胞有明显的抑制作用,而且呈浓度依赖性；HOECHST 33342染色结果显示白花丹素对骨肉瘤U2OS 细胞的抑制作用主要是通过促进凋亡来实现；Western blot结果显示白花丹素能够改变p53/MDM2基因表达比例。结论白花丹素通过细胞凋亡途径抑制骨肉瘤细胞系U2OS细胞的生长。Abstract: Objective To explore the effect of plumbagin on the growth of osteosarcoma cell line U2OS and its possible underlying mechanisms. Methods Cell apoptosis of plumbagin against osteosarcoma was studied by CCK-8 assay；morphological changes was studied by HOECHST 33342 staining；the expression of p53 and MDM2 gene was studied by Western blot assay. Results CCK-8 assay showed that plumbagin had an obvious inhibition on osteosarcoma cell line in a dose-dependent manner；HOECHST 33342 staining showed that plumbagin inhibited the growth of osteosarcoma cell line U2OS through the promotion of apoptosis；Western blot assay showed that plumbagin played the role by changing the expression proportion of p53 and MDM2. Conclusion Plumbagin inhibited osteosarcoma cell line U2OS cells growth through cell apoptotic pathways.
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