Research of kallikrein7 Gene Expression in Different Prostate Tissue
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摘要: 目的 通过测定组织激肽释放酶7 mRNA（KLK7）在正常前列腺、良性增生前列腺及前列腺癌组织中的表达量，探讨KLK7与前列腺良、恶性病变之间的关系，并阐明其在肿瘤发生发展中的表达状态。方法利用荧光实时定量PCR（quantitative real-time PCR）的方法，检测15例正常前列腺、25例良性增生前列腺和53例前列腺癌组织中KLK7的表达量。比较其在上述前列腺组织中表达量的差异;比较KLK在不同临床分期以及是否存在骨转移的前列腺癌组织间的表达差异。结果KLK7在前列腺癌组织中的平均表达量低于正常或良性病变的前列腺组织，差异有统计学意义（P＜0.05）;在晚期前列腺癌组织中的表达低于局限性前列腺癌，差异有统计学意义（P ＜0.05），在有或无骨转移的前列腺癌组织中的表达差异无统计学意义（P＞0.05）。结论KLK7在前列腺癌组织中呈低表达，在晚期前列腺癌组织中的表达低于局限性前列腺癌，提示该基因可能是潜在的诊断前列腺癌及判断预后的分子标志。Abstract: Objective To evaluate the expression of KLK7 mRNA（KLK7） in prostate tissues of different pathology including normal prostate,BPH and prostate cancer,in order to discuss the relationship of this gene with the benign and malignant prostate diseases,and to demonstrate the relationship of its expression level with the occurrence and development of the tumor. Methods Quantitative real-time PCR was used to quantify the KLK7 expression in 15 cases from normal prostate tissues,25 cases of BPH as well as 53 cases of prostate cancer.We compared its different expression between benign and malignant prostate tissues;different clinical tumor stage;with or without bony metastasis. Results We found a significant decrease in KLK7 expression in prostate cancer compared with their normal or benign counterparts (P＜0.05),this difference was also observed in late stage prostate cancer compared to local ones,and there was statistical significance(P＜0.05);its expression level had no significant difference between tissues with or without bony metastasis(P＞0.05). Conclusion KLK7 expresses lower in prostate cancer tissues and in later stage ones,it suggests this gene is hopeful to be the molecular marker for diagnosis and prognosis estimation of prostate cancer.
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Key words:
- Prostate cancer /
- KLK7 /
- real-time PCR /
- Molecular marker
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