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慢病毒介导ITGB4 shRNA对H460SM细胞增殖的抑制作用

陈艳, 周永春, 金从国, 伍治平, 刘馨, 陈晓群, 李佳, 王熙才

陈艳, 周永春, 金从国, 伍治平, 刘馨, 陈晓群, 李佳, 王熙才. 慢病毒介导ITGB4 shRNA对H460SM细胞增殖的抑制作用[J]. 肿瘤防治研究, 2012, 39(09): 1070-1075. DOI: 10.3971/j.issn.1000-8578.2012.09.007
引用本文: 陈艳, 周永春, 金从国, 伍治平, 刘馨, 陈晓群, 李佳, 王熙才. 慢病毒介导ITGB4 shRNA对H460SM细胞增殖的抑制作用[J]. 肿瘤防治研究, 2012, 39(09): 1070-1075. DOI: 10.3971/j.issn.1000-8578.2012.09.007
Chen Yan, Zhou Yongchun, Jin Congguo, Wu Zhiping, Liu Xin, Chen Xiaoqun, Li Jia, Wang Xicai. Inhibition of Lentivirus-mediated ITGB4 shRNA on H460SM Cell Proliferation[J]. Cancer Research on Prevention and Treatment, 2012, 39(09): 1070-1075. DOI: 10.3971/j.issn.1000-8578.2012.09.007
Citation: Chen Yan, Zhou Yongchun, Jin Congguo, Wu Zhiping, Liu Xin, Chen Xiaoqun, Li Jia, Wang Xicai. Inhibition of Lentivirus-mediated ITGB4 shRNA on H460SM Cell Proliferation[J]. Cancer Research on Prevention and Treatment, 2012, 39(09): 1070-1075. DOI: 10.3971/j.issn.1000-8578.2012.09.007

慢病毒介导ITGB4 shRNA对H460SM细胞增殖的抑制作用

基金项目: 国家自然科学基金资助项目(81060177);云南省应用基础研究基金资助项目(2009CD181);中国人事部留学人员科技活动项目择优资助启动基金资助项目(人社厅[2010]412号);云南省卫生科技资助项目(2009NS079)
详细信息
    作者简介:

    陈艳(1973-),女,硕士,副研究员,主要从事肿瘤分子生物治疗、肿瘤复发转移机制的研究

    周永春(1976-),女,硕士,主治医师,主要从事肺癌的基础与临床研究(*:并列第一作者)

    通讯作者:

    王熙才,E-mail:wangxc2005323@126.com

  • 中图分类号: R734.2;R73-35;R73-37

Inhibition of Lentivirus-mediated ITGB4 shRNA on H460SM Cell Proliferation

  • 摘要: 目的 体外实验研究β4整合素shRNA对非小细胞肺癌H460SM细胞增殖的抑制作用,为非小细胞肺癌早期诊断和治疗提供新的靶点。方法 实时定量RT-PCR验证β4整合素表达水平的变化,显微镜下观察细胞形态的变化。通过细胞计数和MTS实验分析抑制β4整合素表达对H460SM细胞增殖的影响;流式细胞术分析抑制β4整合素表达对H460SM细胞凋亡和细胞周期的影响。结果与阴性对照组(H460SM -NS)比较,稳定表达β4整合素shRNA的实验组(H460SM-68、H460SM-71) 细胞β4整合素表达水平明显降低(P<0.01);与阴性对照组比较,实验组细胞的增殖受到明显抑制(P<0.05);实验组细胞凋亡率明显高于阴性对照组,差异有统计学意义(P<0.01);实验组细胞增殖指数PI明显低于阴性对照组(P<0.05),G0/G1期细胞数明显高于阴性对照组(P<0.05),提示抑制β4整合素的表达,细胞周期可能被阻滞在G1/S检测点,导致细胞增殖受到抑制。结论抑制β4整合素的表达,可以抑制非小细胞肺癌H460SM细胞增殖,并可能诱导H460SM细胞凋亡。癌细胞增殖受到抑制可能与细胞周期调控有关。抑制β4整合素的表达,可能不影响非小细胞肺癌细胞凋亡。

     

    Abstract: Objective To investigate the effect of β4 integrin gene silencing on the proliferation of human lung carcinoma variant cell line H460SM was investigated in vitro to further provide new targets for early diagnosis and treatment of non-small cell lung cancer. Methods β4 integrin gene expression was detected by quantitative reverse transcriptase PCR,and the cellular morphology was identified by microscope.Tumor cells growth was detected by MTS assay in vitro.Furthermore,the changes of cell cycle and apoptosis were analyzed by flow cytometry. Results Compared with negative control of shRNA-transduced H460SM cells(H460SM-NS),β4 integrin gene expressions in two different H460SM cells with integrin β4-specific shRNA(H460SM68 and H460SM71) were statistically down-regulated (P<0.01).The growth rates of H460SM cells with stably expressing integrin β4-specific shRNA significantly were decreased compared with negative control(P<0.05).Knockdown of β4 integrin by two different shRNAs led to significantly increase in apoptotic rate as compared with negative control(P<0.01).The proportion of H460SM cells with integrin β4-specific shRNA in the G0/G1 phase was significantly higher than that of negative control,suggesting that the cell proliferation was inhibited as blocking at G1/S phase(P<0.05).PI value of H460SM cells with integrin β4-specific shRNA was also lower than that of negative control(P<0.05). Conclusion Knockdown of β4 integrin expression in non-small cell lung cancer cells could inhibit in vitro cell proliferation.Furthermore,β4 integrin shRNA could induced cell apoptosis in H460SM cells.The possible mechanism of H460SM cell growth inhibition resulted from β4 integrin might be related to cell cycle arrest.

     

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出版历程
  • 收稿日期:  2011-11-10
  • 修回日期:  2012-03-27
  • 刊出日期:  2012-09-24

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