Radiosensitization Effect of Arsenic Trioxide on Human Esophageal Cancer Cell Line EC-1
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摘要: 目的 探讨三氧化二砷(As2O3)对食管癌离体肿瘤细胞EC-1的放射增敏作用，并探讨其机制。方法利用多靶单击数学模型拟合放射剂量-细胞存活曲线，观察As2O3对食管癌细胞株EC-1的放射增敏作用。流式细胞法观察As2O3对细胞周期分布及细胞凋亡的影响。结果As2O3对食管癌细胞株EC-1有明显的放射增敏效应，3 μmol/L As2O3作用48 h的放射增敏比为1.285。药物作用后G2／M期细胞比例增加，G0/G1期和S期细胞比例减少；细胞凋亡指数增加，与对照组相比，差异有统计学意义（P<0.05）。结论As2O3对食管癌细胞株EC-1有明显的放射增敏效应。放射增敏的机制可能与As2O3抑制EC-1细胞的修复能力、使细胞周期阻滞在G2／M期、G0/G1期和S期细胞减少以及凋亡增加有关。
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关键词:
- 三氧化二砷 /
- 人食管癌细胞株EC-1 /
- 放射增敏
Abstract: Objective To explore the radiosensitivity effect and mechanism of action of Arsenic trioxide(Arsenic trioxide ，As2O3) on esophageal carcinoma EC-1 cell line.MethodThe radiosensitivity effect of Arsenic trioxide(As2O3) on esophageal carcinoma EC-1 cell line by fitting the irradiation dose-survival curve using ‘multitarget click mathematical model'.Flow cytometry(FCM) was used to detect the changes of cell apoptosis and cell cycle. Results As2O3 had an obvious radiosensitivity effect on esophageal carcinoma EC-1 cells.After treatment with 3 μmol／L As2O3 for 48 hours，the sensitization enhancement ratio(SER) is 1.285.The G2/M phase ratio is increased while the G0/G1 phase and the S phase ratio decreased after treatment.The apoptotic index was significantly increased(P<0.05),compared with the control group. Conclusion As2O3 has an obvious radiosensitivity effect on esophageal carcinoma EC-1 cells，and the mechanism of action may be related to decreased repair capacity of the EC-1 cells,the cell cycle arresting is G2/M phase,the decreased cell number of G0,G1 and S phase and increased apoptosis.-
Key words:
- As2O3 /
- Human Esophageal Carcinoma EC-1 Cell Line /
- Radiosensitivity
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