Expression of VEGF-C in Nonmucinous Bronchioalveolar Carcinoma and Pulmonary Adenocarcinoma Tissues
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摘要: 目的 探讨非黏液型细支气管肺泡癌侵袭转移能力。方法收集43例非黏液型细支气管肺泡癌新鲜手术标本为实验组，对照组包括黏液型细支气管肺泡癌17例、肺腺癌22例和癌旁肺组织10例；分别采用免疫组织化学法和半定量RT-PCR检测VEGF-C蛋白和mRNA表达。结果非黏液型细支气管肺泡癌组织中VEGF-C的阳性表达率显著高于癌旁肺组织和黏液型细支气管肺泡癌组织(P<0.01），但与肺腺癌组织相比差异无统计学意义(P>0.05）。非黏液型细支气管肺泡癌组织中淋巴结转移率显著高于黏液型细支气管肺泡癌组织(P<0.01），但与肺腺癌组织相比差异无统计学意义(P>0.05 ），非黏液型细支气管肺泡癌组织中20例有淋巴结转移组VEGF-C蛋白的阳性率明显高于23例无淋巴结转移组(P<0.05）。非黏液型细支气管肺泡癌组织中VEGF-C mRNA表达显著高于癌旁肺组织及黏液型细支气管肺泡癌组织(P<0.01），但显著低于肺腺癌组织(P<0.01）。结论 VEGF-C蛋白表达上调提示非黏液型细支气管肺泡癌可能具有与肺腺癌相同的侵袭转移特性；非黏液型细支气管肺泡癌较黏液型细支气管肺泡癌更易发生淋巴结转移。
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关键词:
- 非黏液型细支气管肺泡癌 /
- VEGF-C /
- 转移 /
- 侵袭性
Abstract: Objective To discuss invasiveness and metastasis of nonmucinous bronchioalveolar carcinoma. Methods Fourty-three cases with nonmucinous bronchioalveolar carcinoma were enrolled in experimental group，control group consisted of 17 cases of mucinous bronchioalveolar carcinoma，22 cases of pulmonary adenocarcinoma and 10 cases of lung tissue adjacent to nonmucinous bronchioalveolar carcinoma.Expression of VEGF-C protein and mRNA were detected using immunohistochemical method and RT-PCR，respectively. Results Positive rate of VEGF-C protein in nonmucinous bronchioalveolar carcinoma was significantly higher than that in lung tissue adjacent to nonmucinous bronchioalveolar carcinoma and mucinous bronchioalveolar carcinoma (P<0.01)，compared with pulmonary adenocarcinoma，the difference showed no statistical significance(P>0.05），node metastasis rate in nonmucinous bronchioalveolar carcinoma was significantly higher than that in mucinous bronchioalveolar carcinoma(P<0.01)，compared with pulmonary adenocarcinoma，the difference showed no statistical significance(P>0.05）.Twenty cases of nonmucinous bronchioalveolar carcinoma exhibited node metastasis，compared with non-metastasis group ，positive rate of VEGF-C protein showed statistical significance(P<0.05).Expression of VEGF-C mRNA in nonmucinous bronchioalveolar carcinoma was significantly higher than that in lung tissue adjacent to nonmucinous bronchioalveolar adenocarcinoma and mucinous bronchioalveolar carcinoma(P<0.01)，compared with pulmonary adenocarcinoma，it was dramatically lower(P<0.01). Conclusion Up-regulation of VEGF-C protein in nonmucinous bronchioalveolar carcinoma suggests that it may has similar invasiveness and metastasis characteristic with pulmonary adenocarcinoma，compared with mucinous bronchioalveolar carcinoma，node metastasis more easily occured in nonmucinous bronchioalveolar carcinoma.-
Key words:
- Nonmucinous bronchioalveolar carcinoma /
- VEGF-C /
- Metastasis /
- Invasiveness
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