Effects of Cdx2 Overexpression on Growth and Metastasis of Transplanted Tumor of Human Gastric Cancer in Nude Mice
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摘要: 目的 观察Cdx2基因过表达对裸鼠人胃癌移植瘤生长和转移的影响。方法利用脂质体将pCMV-Cdx2-HA质粒或pCMV-HA质粒分别转染人胃癌MGC-803细胞，命名为MGC-803/Cdx2细胞或MGC-803/EV细胞。将两种细胞分别注射入裸鼠近腋右背侧皮下，待皮下成瘤后将瘤组织接种于胃，建立裸鼠人胃癌移植瘤模型：接种MGC-803/Cdx2皮下瘤的裸鼠为实验组，接种MGC-803/EV皮下瘤的裸鼠为对照组；30 d后处死裸鼠，观察移植瘤生长、转移情况，并应用半定量逆转录-聚合酶链反应(RT-PCR）和Western blot技术检测移植瘤中Cdx2 mRNA和蛋白的表达。结果实验组肿瘤体积为(13.42 ±2.34）mm3，明显低于对照组的肿瘤体积(17.59±2.80）mm3(P<0.05）；实验组成瘤率(73.3%）低于对照组成瘤率(86.7%）；两组肿瘤转移情况比较，差异无统计学意义(P>0.05）；实验组肿瘤组织Cdx2 mRNA和蛋白表达明显增加(P<0.05）。结论 Cdx2过表达抑制裸鼠人胃癌移植瘤的生长，但对肿瘤转移无明显影响。Abstract: Objective To study the effects of Cdx2 overexpression on growth and metastasis of transplanted tumor of human gastric cancer in nude mice. Methods The recombined eukaryotic expression vector pCMV-Cdx2-HA or empty vector pCMV-HA was transfected into gastric cancer MGC-803 cells individually by Lipofectamine，which was named as MGC-803/Cdx2 cells or MGC-803/EV cells.BALB/C nude mice were inoculated subcutaneously with MGC-803/Cdx2 cells or MGC-803/EV cells and then the subcutaneous tumor was transplanted into stomach.The nude mice transplanted with MGC-803/Cdx2 tumor were named as the experimental group while the nude mice transplanted with MGC-803/EV tumor were named as the control group.After mice were executed，the volume of transplanted tumor was measured and tumor metastasis was observed.The expression of Cdx2 in tumor tissue was detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Results The volume of transplanted tumor in the experimental group was (13.42±2.34) mm3，which was lower than that in the control group (P<0.05).Meanwhile，the rate of tumor formation in the experimental group (73.3%) was lower than that in the control group (86.7%).The situation of tumor metastasis was not difference between the control group and the experimental group (P>0.05).Compared with the control group，both Cdx2 mRNA and protein were upregulated in the experimental group (P<0.05). Conclusion Cdx2 overexpression inhibites growth of transplanted tumor of human gastric cancer in nude mice，but it has no effect on tumor metastasis.
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Key words:
- Cdx2 /
- Gastric cancer /
- Transplanted tumor
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