Abstract: Objective To investigate the effects of VTMD and PEI-Gal on VP3 gene mediated apoptosis induction and proliferation suppression in murine transplanted Hepatocarcinoma. Methods Forty mice bearing hepatocarcinoma subcutaneously were divided randomly into 4 groups：control group；PEI-Gal+plasmid group;PEI-Gal+plasmid+ultrasound group and PEI-Gal+UTMD+plasmid group.Histological examination、apoptotic index and transfection efficiency were evaluated by the frozen section.the tumor size was measured regularly.The rate of tumor growth inhibition was calculated and the tumor growth curve was described.The protein expressions of Bcl-2 and Caspase-3 were investigated by immunohistochemistry. Results The highest transfection efficiency was observed in PEI-Gal+UTMD+plasmid group which was significantly higher than that in any other groups(P<0.01)and no tissue damage was seen histologically.The tumor inhibition rate(56.6%）and apoptotic index (34.57%±3.56%）were the highest in PEI-Gal+UTMD+pCDNA-VP3 group compared with those control group and PEI-Gal+pCDNA-VP3 group.The protein expression of Bcl-2 was down-regulated markedly，whereas Caspase-3 was up-regulated remarkedly(P<0.01). Conclusion UTMD associated with PEI-Gal can enhance efficiency of gene transfection significantly.The method associated with VP3 gene can significantly induce apoptosis and inhibit proliferation of transplanted tumors in mice.