Expression of D2-40 and survivin in Cervical Carcinoma and Their Relation to Prognosis
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摘要: 目的 研究D2-40和生存素（survivin）在宫颈癌中的表达及与预后的相关性。 方法采用免疫组织化学方法检测97例宫颈癌中D2-40、survivin的表达，对D2-40标记的LVD进行图像分析。对有随访结果的56例作单、多因素相关生存分析。研究设宫颈上皮内瘤变、慢性炎作为对照组。 结果D2-40标记的LVD值及survivin表达在三组中差异均有统计学意义(P<0.01)。D2-40、survivin在宫颈癌中的表达与浸润深度、组织学类型、FIGO分期及淋巴结转移呈正相关（P<0.01）。D2-40的表达与survivin蛋白的表达呈正相关(P<0.01)。在随访的56例中，单因素分析显示:宫颈癌中D2-40标记的LVD、survivin表达、浸润深度、组织学类型、FIGO分期及淋巴结转移均与患者生存率有关，多因素Cox比例风险分析显示除浸润深度外均具有独立的预后意义。结论D2-40在宫颈癌组织中高表达，提示D2-40与宫颈癌的发生、发展及转移密切相关。D2-40的表达与宫颈癌组织中survivin蛋白的表达密切相关。两者均是影响宫颈癌预后的因素，并有独立的预后意义。Abstract: Objective To investigate the expression of D2-40 and survivin protein in cervical carcinoma and its relation to prognosis. Methods The expression of D2-40 and survivin protein in 97 cases of cervical carcinoma were investigated immunohistochemically，and average LVD marked by D2-40 antibody were examined by computer.Fifty six cases were followed up，in which the prognosis was studied with both univariate and multivariate analysis.The cases of cervical intraepithelial neoplasia，chronic cervicitis were compared in the investigation. Results The average LVD marked by D2-40 and expression of survivin protein were obviously different among three groups (P<0.01).D2-40 and survivin protein expression were associated with differentiated grade，aggressive degree，FIGO stage and lymph nodal involvement (P<0.01).The expression of D2-40 was positively associated with survivin protein (P<0.01).In the 56 cases with followed-up data，univariant analysis revealed that the average LVD marked by D2-40，expression of survivin，differentiated grade，aggressive degree，FIGO stage and lymph nodal involvement were correlated with the survival rate.Cox stepwise proportional hazards analysis showed that they were prognostic factors independently except aggressive degree. Conclusion D2-40 was highly expressed in cervical carcinoma，which suggested that D2-40 was closely related to the growth，development and metastasis of cervical carcinoma.And there was intimate correlation between expression of D2-40 and survivin protein in cervical carcinoma.They were independent prognostic factors independently.
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Key words:
- D2-40 /
- survivin /
- Cervical carcinoma /
- Immunohistochemistry /
- Prognosis
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