Expression and Significance of miR-106a and miR-24-1 in Colon Cancer
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摘要: 目的 检测结肠癌与癌旁组织miR-106a和miR-24-1的表达差异及其与c-myc的表达是否具有关联性。方法选临床病理诊断明确的结肠癌患者，手术后取癌组织标本提取RNA，RT-PCR检测原癌基因c-myc在结肠癌组织与癌旁组织中的表达。TaqMan荧光定量PCR检测结肠癌与癌旁组织miR-106a和miR-24-1的表达差异。结果c-myc表达阳性的癌组织表达miR-106a要明显高于癌旁组织，其差异具有统计学意义（P<0.05）;c-myc表达阴性的癌组织表达miR-106a要高于癌旁组织（P<0.05），结肠癌组织与癌旁组织miR-24-1的表达无差异。结论结肠癌组织c-myc阳性组和c-myc阴性组表达miR-106a均高于癌旁组织，miR-106a有可能作为结肠癌的筛查目标之一；结肠癌组织c-myc阳性组和c-myc阴性组表达miR-24-1与癌旁组织无差异，还需更多研究证实其与结肠癌的关系。Abstract: Objective To investigate the expression of miR-106a and miR-24-1 in both colon cancer and adjacent tissues，and analyze the relationship between the expression of miR-106a，miR-24-1 and c-myc gene. Methods To extract RNA from colon cancer tissues and their adjacent ones，detected the mRNA expression of c-myc by RT-PCR.The TaqMan fluorescence quantitative PCR was used to analyze the expression of the miR-106a and miR-24-1 in both colon cancer and adjacent tissues. Results The results showed that the expression of miR-106a in cancer tissues was higher than that in the adjacent tissues in the c-myc positive group（P<0.05），and the expression of miR-106a in cancer tissues was also higher than that in the adjacent tissues in the c-myc negative ones(P<0.05).The expression of miR-24-1 showed no difference either between cancer and adjacent tissues，or between positive and negative c-myc control. Conclusion The expression of miR-106a in both c-myc positive group cancer tissues and the c-myc negative ones were higher than that in adjacent tissues.miR-106a might be a blood screening target of colon cancer.There was no difference of the expression of miR-24-1 in both the c-myc positive group ones and the c-myc negative ones，which need more research to prove the relation between miR-24-1 and colon cancer.
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Key words:
- Colon cancer /
- miR-106a /
- miR-24-1 /
- c-myc
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