Abstract: ObjectiveTo explore the gene transfection efficiency of folic acid/polyamide-amine as miR-7 vector and its targeting ability for glioma,so as to lay the foundation to develop an efficient delivery of small molecule drugs targeting glioma. Methods FA/PAMAM comoles compound was prepared by dialysis method.The transmission electron microscope was performed to observe the morphology of the nanoparticles.After transfecting miR-7 gene into U251 glioma cell line,fluorescence microscope was used to detect the gene transfection efficiency,quantitative RT-PCR was used to detect the miR-7 level.The intracranial glioma model was established in de-thymus mice,the nanoparticles were transplanted by the way of vena caudalis,internal carotid artery,and tumor situ.After 48 hours,the frozen section was obtained to observe the aggregation extent.And immunocytochemistry and western blot methods were used to test the protein expression of EGFR and PCNA. Results The nanoparticle,with sphere morphology,may transfer efficiently the miR-7 gene into U251 glioma cells,and increase the miR-7 level.And it can be found in tumor situ,in spite of by different transplant ways.The aggregation extent was the vena caudalis way less than the internal carotid artery way,and furthermore less the tumor situ way (P<0.05).The protein level of EGFR and PCNA in FA/PAMAM/miR-7 group both lower than the control group(P<0.05). Conclusion FA/PAMAM can transfect effectively miR-7 into glioma both in vivo and in vitro,and is expected to become an efficient delivery of small molecule drugs targeting glioma.