Abstract: ObjectiveTo investigate the effects of oxidative stress on colon cancer cell migration, vascular epithelial growth factor expression and gap junction intercellular communication. MethodsWe stimulated HCT-8 human colon cancer cell with different concentration of hydrogen peroxide, through which mimicking the oxygen stress environment in the colon lumen was devedoped. We observed the colon cancer cell migration using Transwell Chamber assay, the expression of VEGF gene using immunocytochemistry, the gap junction intercellular communication using the scrape loading/dye transfer technique, and furthermore the expression of intercellular communication protein Connexin 43 using Western blot. ResultsThe oxygen stress environment induced by hydrogen peroxide at concentration ≤10-5mol/L shightly promoted and concentration of 10-5mol/L significantly promoted the colon cancer migration (t=2.247, P<0.05), up-regulated the expression of VEGF gene (Z=3.938, P=0.000) and inhibited the intercellular communication (Z=3.938, P=0.000). ConclusionOxidative stress mimicked by hydrogen peroxide might be involved in colorectal tumor development through promotion of the colon cancer cell migration, up-regulation of VEGF gene and inhibition of the intercellular communication.