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间隙连接蛋白Cx26和Cx43的表达及与食管鳞癌浸润和转移关系

Expression of Gap Junction Proteins Connexin26 and Connexin43 and Their Correlation with Occurence,Invasion and Metastasis in Esophageal Squamous Cell Carcinoma

  • 摘要: 目的探讨间隙连接蛋白Cx26和Cx43的表达在食管鳞状细胞癌发生、发展以及浸润和转移中的作用。方法通过RT-PCR、免疫组织化学技术联合检测Cx26和Cx43在42例食管鳞状细胞癌组织、17例癌旁不典型增生组织以及42例相应正常食管黏膜组织的表达情况。结果在食管鳞癌组织、癌旁不典型增生组织及正常食管黏膜中Cx26、Cx43 mRNA相对表达量及蛋白阳性表达率均低于正常食管黏膜和癌旁不典型增生组织。Cx26、Cx43蛋白和mRNA阳性表达率均随癌组织浸润深度的加深而明显降低,在有淋巴结转移的癌组织均明显低于无淋巴结转移组癌组织。Cx26、Cx43在食管鳞癌组织中mRNA相对表达量均随分化程度降低呈下降趋势。Cx26与Cx43 mRNA表达和蛋白表达之间均呈正相关关系。结论Cx26、Cx43基因抑制食管鳞癌发生、发展及浸润、转移,是早期预测食管鳞癌发生和浸润转移的有效指标。

     

    Abstract: ObjectiveTo study the relationship between the expression of gap junction proteins connexin26 and connexin43 and the occurence,invasion and metastasis of esophageal squamous cell carcinoma. MethodsThe expression of Cx26 and Cx43 proteins and mRNA were detected by SP immunohistochemical and RT-PCR methods in 42 cases of esophageal squamous cell carcinoma (EC) tissue,17 cases of mucosa adjacent to cancer and 42 cases of normal esophageal mucosa. ResultsThe positive expressions of Cx26, Cx43 protein and mRNA in cancer tissue were both lower than that in normal mucosa tissue, and the positive expression of Cx26, Cx43 protein in tumor-adjacent atypical hyperplasia epithelium had a decreasing trend. Cx26, Cx43 gene loss or decreased expression had closely correlation with invasion depth and lymph node metastasis of esophageal squamous cell carcinoma. There was a correlation between the expression of Cx26, Cx43 protein and differentiation levels of tumor, and positive correlation between the expression of Cx26,Cx43 protein and the expression of Cx26, Cx43 mRNA. ConclusionConnexin26 and connexin43 can provide a theoretical basis to suppress of carcinogenesis, invasion and metastasis of esophageal squamous cell carcinoma and may be used for early diagnosis, invasion and metastasis of esophageal squamous cell carcinoma.

     

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