脂质体阿霉素热化疗对食管癌细胞的毒性实验研究

张振华; 吴敬波

doi:10.3971/j.issn.1000-8578.2011.07.002

脂质体阿霉素热化疗对食管癌细胞的毒性实验研究

Cytotoxicity Effect of Thermo-chemotherpy with Pegylated Liposomal Doxorubicin on Human Esophageal Carcinoma in vitro

摘要

摘要: 目的了解脂质体阿霉素体外不同温度热化疗对食管癌细胞株的细胞毒性作用。方法以体外培养食管癌细胞株EC9706为靶细胞，以游离阿霉素作为阳性对照，未加药物组作为阴性对照，采用WST-1法测定阿霉素和脂质体阿霉素对EC9706的半数抑制浓度(IC50)。再使用此IC50时药物的浓度，在37℃、41℃、43℃下水浴加温1 h进行热化疗，同样使用WST-1法测定其细胞杀伤率。结果阿霉素、脂质体阿霉素对食管癌细胞株EC9706的IC50分别为19.064 μg/ml、51.359 μg/ml(含阿霉素的量为5.707 μg/ml)。在37℃条件下，阿霉素与脂质体阿霉素对EC9706细胞株的杀伤率分别为(49.29±2.14)%、(49.39±6.07)%（P>0.05）。在41℃条件下，单纯加热、阿霉素、脂质体阿霉素对EC9706的杀伤率分别为(25.25±2.52)%、(64.80±5.01)%、(76.39±3.42)%，两种药物加热条件下的细胞杀伤作用均较单纯加热组高(P<0.01)，且脂质体阿霉素较阿霉素的细胞毒作用提高了11.59% (P<0.01)。在43℃条件下，虽脂质体阿霉素的杀伤作用(79.05 ± 3.09)%较阿霉素(71.89±2.30)%提高了7.16% (P<0.05)，但与该药在41℃条件下的杀伤作用比较仅提高2.66%，且差异没有统计学意义(P>0.05)。结论食管癌细胞株EC9706对阿霉素和脂质体阿霉素都很敏感。在热化疗作用上，阿霉素和脂质体阿霉素对食管癌细胞较单纯热疗具有更强的杀伤作用；在37℃条件下，脂质体阿霉素对细胞的杀伤作用与阿霉素相当，在41℃和43℃条件下脂质体阿霉素对细胞的杀伤作用强于阿霉素，但脂质体阿霉素在43℃与41℃对细胞的杀伤作用相当，两种药物联合热疗都具有协同增敏作用。

Abstract: ObjectiveTo observe the cytotoxicity effect of thermo-chemotherapy with pegylated liposomal doxorubicin (PLD) on human esophageal squamous carcinoma cell line (EC9706). MethodsThe EC9706 cell line was treated, and cytotoxicity effect was observed with WST-1 assay, the free doxorubicin(ADM), and no drug group was positive control and negative control, respectively. Firstly, the 50% inhibiting concentration(IC50) of PLD and ADM was measured. Then using the IC50, the thermo-chemotherapy effect was measured after 37℃，41℃，43℃ 1 hour by a water bath heating system, respectively. ResultsThe average IC50 of ADM and PLD on EC9706 cell line was 19.064 μg/ml and 51.359 μg/ml(containing doxorubicin was 5.707 μg/ml), respectively. On 37℃, the cytotoxicity effect of ADM and PLD on EC9706 was (49.29±2.14)% and (49.39±6.07)%, respectively. There was no statistical significance(P>0.05); on 41℃, the effect of hyperthermia alone, ADM and PLD was (25.25±2.52)%，(64.80±5.01)% and (76.39±3.42)%，respectively. The showed the effect of the two drug was superior to hyperthermia alone(P<0.01), and PLD was superior to ADM(P<0.01), enhanced 11.59%, on 43℃, the effect of PLD (79.05±3.09)% was superior to ADM (71.89±2.30%)(P<0.05) which enhanced 7.16%, but in comparison with 41℃of PLD which enhanced only 2.66%.There was no statistical significance (P>0.05). ConclusionADM and PLD on EC9706 is superior to hyperthermia alone; on 37℃, the cytotoxicity effect of PLD is equal to ADM, on 41℃ and 43℃, the effect of PLD is superior to ADM, but the effect of PLD on 41℃ is equal to that on 43℃.

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