慢病毒介导的siRNA干扰乳腺癌MCF-7细胞VEGF-C表达的实验

Down-regulation of Vascular Endothelial Cell Growth Factor-C Expression in MCF-7 Cells with Lentivirus-mediated Small Interfering RNA Vectors

摘要: 目的研究慢病毒载体介导的siRNA对乳腺癌MCF-7细胞系VEGF-C表达的敲减作用。方法构建慢病毒VEGF-C/siRNA载体，转染乳腺癌MCF-7细胞，采用Real-time PCR检测MCF-7细胞在转染前后VEGF-C的mRNA表达，计算其转染效率和VEGF-C敲减率。结果慢病毒VEGF-C/siRNA转染效率超过80%，其VEGF-C的mRNA表达敲减率达50%。结论慢病毒VEGF-C/siRNA转染率高，能有效敲减VEGF-C的mRNA表达。

Abstract: ObjectiveTo study the reduction of VEGF-C expression of MCF-7 cells interfered with lentivirus-mediated small interfering RNA vectors. MethodsLentivirus- mediated small interfering RNA vectors were constructed and transfected into breast cancer MCF-7 cells. Real-time PCR was performed to detect the expression of VEGF-C. The proportion of transfected cells and the knock-down rate of VEGF-C were calculated. ResultsThe transduction rate of Lentivirus-mediated small interfering RNA was more than 80%. mRNA expression of VEGF-C decreased nearly 50%. ConclusionLentivirus-mediated small interfering RNA vectors had a high transfection rate and might reduce the expression of VEGF-C significantly.