NTx在小细胞肺癌骨转移裸鼠模型中疗效的预测作用
Prognostic Effects of Serum Cross-linked N-telopeptide of Type Ⅰ Collagen in Skeletal Metastasis Model of Small Cell Lung Cancer
-
摘要: 目的探讨溶骨性骨代谢生化指标血Ⅰ型胶原交联氨基末端肽（cross-linked N-telopeptide of typeⅠcollagen，NTx）的定量测定对肺癌骨转移治疗的意义。方法将18只Balb/c-nu/nu雄性裸鼠随机分成正常组、实验组和对照组。将人肺小细胞肺癌细胞株H446种植于实验组和对照组裸鼠右后肢胫骨，制成小细胞肺癌骨转移裸鼠动物模型，第一次服氯磷酸二钠前及处死前半小时各留血样1次，每1周测体重，观察存活情况，给药6周后处死裸鼠, 所有裸鼠行放射学检查并取各裸鼠的右后肢行病理分析，用ELISA方法测定血清NTx水平。结果放射学及病理检查显示裸鼠接种H446细胞后引起明显的肿瘤性骨损伤。实验组、对照组裸鼠血清NTx高于正常组裸鼠血清NTx (P< 0. 01)，实验组裸鼠经氯膦酸二钠治疗前后血清NTx水平差异有统计学意义(P< 0. 01)。结论定量测定NTx水平变化可用于预测氯膦酸二钠治疗小细胞肺癌骨转移的疗效。血清NTx对小细胞肺癌骨转移的诊断有重要参考意义，可协助及时诊断恶性肿瘤骨转移，且氯膦酸二钠可明显减轻小细胞肺癌骨转移程度。Abstract: ObjectiveTo establish a skeletal metastasis model of small cell lung cancer, and evaluate the prognosis role of serum cross-linked N-telopeptide of typeⅠcollagen(NTx) in disodium clodronate against skeletal metastasis. MethodsA skeletal metastasis model was established by injection of small cell lung carcinoma line(SCLC) H446 cells and disodium clodronate was given to the animals once radiologically osteolytic metastasis was observed. Both X-ray and histological assay were applied to diagnose the bone metastasis. The levels of serum NTx were measured by using ELISA. ResultsBoth X-ray and histological assay showed markedly bone injuries after injection cells into the right leg of nude mice.The level of serum NTx in the nude mice with bone metastasis was significantly higher than those without bone metastasis (P<0.01), and it was also significantly different in the nude mice with disodium clodronate. ConclusionSerum NTx played a biomarker role of bone metastasis with malignant carcinoma through a timely diagnosis. Disodium clodronate might be an appropriate treatment of bone metastasis.
下载:
