Abstract: ObjectiveTo study the 21 genotypes of human papilloma virus(HPV) and its clinical significance. Methods 1 540 females with cervical disease were carried through HPV typing in all 21 genotypes in our hospital,then comparing the different genotypes and clincal disease feature. Results The total positive rate was 31.8%(489/1 540),21 isoforms were checked out， HPV16,58,31 were seen mostly in high risk and HPV6,11 were seen mostly in low risk.Among the positive rates in different stage of years,the less than 40 years old group was the highest.Positive for HPV infection in cervical lesions and cervical carcinoma prevalence was significantly higher than that in the negative group. Conclusion Infection of HPV genotypes are related closely with cervical different-grade lesions,which may apply reasonable evidences for screening,prevention and cure of cervical carcinoma.