肝细胞生长因子对结肠癌细胞SW620增殖、侵袭的影响

Effects of Hepatocyte Growth Factor on Proliferation and Migration of Human Colon Cancer Cells SW620

摘要: 目的探讨肝细胞生长因子（Hepatocyte growth factor，HGF）对人结肠癌细胞株SW620的增殖和侵袭能力的影响。方法应用MTT法分析HGF对SW620细胞增殖的影响，确定最佳促增殖浓度；应用基底膜重建实验和电镜观察HGF对细胞侵袭能力的影响；应用PCR技术检测HGF对MMP-2 mRNA表达的影响。结果当HGF浓度≥10ng/ml时促进SW620增殖且呈质量浓度依赖性；HGF明显促进SW620 移行且呈质量浓度依赖关系；加入 HGF 后，SW620细胞内的微丝明显增多，MMP-2表达水平升高。结论HGF可以在体外促进人结肠癌细胞SW620的增殖和移行。

Abstract: ObjectiveTo investigate the effects of Hepatocyte growth factor (HGF) on the proliferation and migration of SW620. Methods MTT was used to assess the effect of HGF on the proliferation of SW620.The effect of HGF on the migration of cells was calculated by restoration of basal membrane；the ultrastructure of SW620 after being treated with HGF was observed by TEM；the expression of MMP-2 was determined by PCR. Results HGF promoted both the proliferation and migration of SW620 in a dose-dependent manner.Microfilaments increased significantly after being treated with HGF.HGF up-regulated the expression level of MMP-2 mRNA of SW620 cells. Conclusion HGF can promote the proliferation and migration of colon cancer cell SW620.