姜黄素对人鼻咽癌CNE-2Z细胞增殖及 凋亡的影响

范德生; 甄蕾; 孙宁

doi:10.3971/j.issn.1000-8578.2011.03.004

姜黄素对人鼻咽癌CNE-2Z细胞增殖及凋亡的影响

1.200021 上海，上海中医药大学附属曙光医院病理科；2.同济大学医学院；3.广东医学院病理学教研室

详细信息

中图分类号: R793.63
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出版历程
- 收稿日期: 2010-02-14
- 修回日期: 2010-08-31
- 刊出日期: 2011-03-24

Effect of Curcumin on Proliferation and Apoptosis of Human Nasopharyngeal Carcinoma CNE-2Z Cells

1. Department of Pathology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021，China; 2. College of Medicine, Tongji University; 3. Department of Pathology, Guangdong Medical College

摘要

摘要: 目的探讨姜黄素对人鼻咽癌CNE-2Z细胞增殖及凋亡的影响及其相关的分子机制。方法体外培养的CNE-2Z细胞经不同剂量姜黄素处理后，用MTT法观察对细胞增殖的影响，流式细胞术检测细胞周期和凋亡率的改变，Western blot检测姜黄素处理后相关蛋白Bcl-2、Bax表达水平的变化。结果姜黄素对CNE-2Z细胞的生长和增殖都具有一定程度的抑制作用，阻断细胞于S期和G2/M期。姜黄素处理后CNE-2Z细胞凋亡抑制基因Bcl-2蛋白表达减少,同时促凋亡基因Bax蛋白表达增加。结论姜黄素可抑制CNE-2Z细胞增殖并促进其凋亡。
- 关键词：姜黄素 /
- 鼻咽癌 /
- 增殖 /
- 凋亡 /
- Bcl-2 /
- Bax
Abstract: ObjectiveTo study the effects of Curcumin on proliferation and apoptosis of human nasopharyngeal carcinoma cell CNE-2Z and its possible mechanism. MethodsAfter treatment with Curcumin at various concentrations, the proliferative inhibition of CNE-2Z was observed by MTT assay. The cell cycle influence and cell apoptosis rate were detected by flow cytometry. The expression of Bcl-2 and Bax were detected by Western blot. ResultsCurcumin could inhibit the proliferation of human nasopharyngeal carcinoma cells CNE-2Z at a time and dose dependent pattern.The cells were blocked at G2/M and S phases. When treated with Curcumin for 24h, the percentage of cell apoptosis was increased. Bcl-2 was down-regulated whereas Bax was up-regulated. ConclusionCurcumin could inhibit the proliferation of CNE-2Z and induce cell apoptosis.
- Key words：Curcumin /
- Nasopharyngeal carcinoma /
- Proliferation /
- Apoptosis /
- Bcl-2 /
- Bax