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姜黄素对人鼻咽癌CNE-2Z细胞增殖及 凋亡的影响

范德生, 甄蕾, 孙宁

范德生, 甄蕾, 孙宁. 姜黄素对人鼻咽癌CNE-2Z细胞增殖及 凋亡的影响[J]. 肿瘤防治研究, 2011, 38(03): 254-256. DOI: 10.3971/j.issn.1000-8578.2011.03.004
引用本文: 范德生, 甄蕾, 孙宁. 姜黄素对人鼻咽癌CNE-2Z细胞增殖及 凋亡的影响[J]. 肿瘤防治研究, 2011, 38(03): 254-256. DOI: 10.3971/j.issn.1000-8578.2011.03.004
FAN De-sheng, ZHEN Lei, SUN Ning. Effect of Curcumin on Proliferation and Apoptosis of Human Nasopharyngeal Carcinoma CNE-2Z Cells[J]. Cancer Research on Prevention and Treatment, 2011, 38(03): 254-256. DOI: 10.3971/j.issn.1000-8578.2011.03.004
Citation: FAN De-sheng, ZHEN Lei, SUN Ning. Effect of Curcumin on Proliferation and Apoptosis of Human Nasopharyngeal Carcinoma CNE-2Z Cells[J]. Cancer Research on Prevention and Treatment, 2011, 38(03): 254-256. DOI: 10.3971/j.issn.1000-8578.2011.03.004

姜黄素对人鼻咽癌CNE-2Z细胞增殖及 凋亡的影响

详细信息
  • 中图分类号: R793.63

Effect of Curcumin on Proliferation and Apoptosis of Human Nasopharyngeal Carcinoma CNE-2Z Cells

  • 摘要: 目的探讨姜黄素对人鼻咽癌CNE-2Z细胞增殖及凋亡的影响及其相关的分子机制。方法体外培养的CNE-2Z细胞经不同剂量姜黄素处理后,用MTT法观察对细胞增殖的影响,流式细胞术检测细胞周期和凋亡率的改变,Western blot检测姜黄素处理后相关蛋白Bcl-2、Bax表达水平的变化。结果姜黄素对CNE-2Z细胞的生长和增殖都具有一定程度的抑制作用,阻断细胞于S期和G2/M期。姜黄素处理后CNE-2Z细胞凋亡抑制基因Bcl-2蛋白表达减少,同时促凋亡基因Bax蛋白表达增加。 结论姜黄素可抑制CNE-2Z细胞增殖并促进其凋亡。

     

    Abstract: ObjectiveTo study the effects of Curcumin on proliferation and apoptosis of human nasopharyngeal carcinoma cell CNE-2Z and its possible mechanism. MethodsAfter treatment with Curcumin at various concentrations, the proliferative inhibition of CNE-2Z was observed by MTT assay. The cell cycle influence and cell apoptosis rate were detected by flow cytometry. The expression of Bcl-2 and Bax were detected by Western blot. ResultsCurcumin could inhibit the proliferation of human nasopharyngeal carcinoma cells CNE-2Z at a time and dose dependent pattern.The cells were blocked at G2/M and S phases. When treated with Curcumin for 24h, the percentage of cell apoptosis was increased. Bcl-2 was down-regulated whereas Bax was up-regulated. ConclusionCurcumin could inhibit the proliferation of CNE-2Z and induce cell apoptosis.

     

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出版历程
  • 收稿日期:  2010-02-14
  • 修回日期:  2010-08-31
  • 刊出日期:  2011-03-24

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