Abstract: Objective To study the association of Src homology 2 domain containing adaptor protein B(SHB) gene with pathogenesis of breast cancer,the single nucleotide polymorphism(SNP) of gene SHB in turnor tissue and henlthy peripheral blood were detected. Methods The tissues of 96 patients with breast cancer were included in a case -control associative study，and the circulating DNA of 96 healthy people were measured at the same time.Sequences of proximal promoter，coding regions (including sequence near splicing site) of SHB gene were detected by PCR amplification and DNA-sequencing. Results A total of 7 SNPs were identified for SHB gene；there were 5 high frequency SNPs and 2 low frequency SNPs.One SNP(P/-1047) located in the promoter of SHB had significant difference of allele and genotype between breast cancer and controls(P<0.05). Conclusion The SNP in the promoter of SHB gene might be related to the occurrence of breast cancer.