Abstract: Objective The lung cancer chemosensitivity associated genes are screened to provide genetic basis for individual therapeutic project. Methods Fresh lung cancer tissue were collected, total tissue RNA were extracted,and tumor drug sensitivity experiment(MTT method) were conducted.All of pathological diagnosis were moderately differentiated squamous cell carcinoma,all of tissues were classified into high-sensitivity group and low-sensitivity group.cDNA array technique was applied to screen differentially expressed genes. Part of differentially expressed genes were assayed by Western blot. Results -Four high-sensitivity cases and 4 low-sensitivity cases under combination of DDP and TAX were selected according MTT, cDNA array result showed that 57 differentially expressed genes existed between high-sensitivity group and low-sensitivity group, including 15 differentially significantly expressed genes.c--myc expressed higher in high-sensitivity group than low-sensitivity group, GST pi expressed higher in low-sensitivity group than high-sensitivity group, which was accord to cDNA array. Conclusion There were differentially expressed genes in lung cancer with different chemosensitivity, these gene might become biomarkers for prediction of chemosensitivity.