Abstract: Objective To investigate the effect of human secondary lymphoid tissue chemokine (SLC) on invasive ability ability of Siha cell line. Methods The SLC gene was amplified from human spleen cDNA，and constructed into eukaryotic expression vector pDsRed2-N1.The plasmid was transfected into human cervical cancer Siha cell line.The tumor invasive ability was detected using transwell，and the activation of NF-κB was tested by NF-κB specific promoter Luciferase reporter system. Results The SLC plasmid was constructed and transfected Siha cell successfully，with PCR，enzyme digestion and ELISA.Siha-CCL21 had obviously increased ability of tumor invasion (2.34±0.14 times) and NF-κB activation (3.98±0.194 times) compared with controls. Conclusion Although as a chemoattractant，the chemokine SLC plays multiple roles，including promotion of NF-κB activation and invasion of cancer cells.