Abstract: Objective To investigate the effects of pseudo CO₂ pneumoperitoneum environment on the growth and metastasis of cervical cancer cells. Methods Cervical cancer cells were exposed to CO₂ environment with different pressure and different time in a pseudo pneumoperitoneum model. The cell growth curve, cell growth cycle,Celladhesive and the cell invasive ability were detected.The changes of growth, invasion and metastasis were observed. Results With CO₂, the cell proliferation ability of cervical cancer cells was obviously enhanced after a short restrained;while it had no relation to the pressure of CO₂ . The cell adhesive and invasive ability of cervical cancer cells were obviously decreased. Conclusion CO₂ environment can enhance the cell proliferation after a short restrained and inhibite the cell adhesive and invasive ability of cervical cancer cells.