Abstract: Objective To investigate the effects of Chitosan/cisplatin drug release films on the cell grouth of peritoneal dissemination gastric cancer in nude mice. Methods The chitosan/cisplatin mixing chitosan and cis-platinum. 32 nude mice inoculated intraperitoneally with gastric cancer cells were divided into four groups.The chitosan/cisplatin films，chitosan membrane, cisplatin and saline were implanted in the mice. Results Chitosan/cisplatinum drug release films showed satisfactory drug loading, drug encapsulation efficiency and in vitro sustained release,Compared with the other 3 groups, Chitosan/cisplatinum drug relense film exhibited significant a decrease in the number of peritoneal nodules (P<0.01), an increased in the survival rates of the nude mice and a extension of survival time (P<0.05), and a decrease in the side effects of the drug(P>0.05). Conclusion Chitosan/cisplatin drug release film can inhibit the peritoneal cells of gastric cancer grown in nude mice and can prolong the survival time of the nude mice.