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RNAi下调hTER长T基因对MCF-7乳腺癌细胞生的影响

曾晓华, 刘长安, 王继见, 印国兵, 郭 丹

曾晓华, 刘长安, 王继见, 印国兵, 郭 丹. RNAi下调hTER长T基因对MCF-7乳腺癌细胞生的影响[J]. 肿瘤防治研究, 2010, 37(07): 763-765. DOI: 10.3971/j.issn.1000-8578.2010.07.008
引用本文: 曾晓华, 刘长安, 王继见, 印国兵, 郭 丹. RNAi下调hTER长T基因对MCF-7乳腺癌细胞生的影响[J]. 肿瘤防治研究, 2010, 37(07): 763-765. DOI: 10.3971/j.issn.1000-8578.2010.07.008
ZENG Xiao-hua, LIU Chang-an, WANG Ji-jian, YIN Guo-bing, GUO Dan. Effect of Targeting hTERT Gene Inhibited in MCF-7 Breast Cancer Cell Growth by RNA Interference[J]. Cancer Research on Prevention and Treatment, 2010, 37(07): 763-765. DOI: 10.3971/j.issn.1000-8578.2010.07.008
Citation: ZENG Xiao-hua, LIU Chang-an, WANG Ji-jian, YIN Guo-bing, GUO Dan. Effect of Targeting hTERT Gene Inhibited in MCF-7 Breast Cancer Cell Growth by RNA Interference[J]. Cancer Research on Prevention and Treatment, 2010, 37(07): 763-765. DOI: 10.3971/j.issn.1000-8578.2010.07.008

RNAi下调hTER长T基因对MCF-7乳腺癌细胞生的影响

详细信息
    通讯作者:

    刘长安

  • 中图分类号: R737.9

Effect of Targeting hTERT Gene Inhibited in MCF-7 Breast Cancer Cell Growth by RNA Interference

More Information
    Corresponding author:

    LIU Chang-an

  • 摘要: 目的:利用siRNA在乳腺癌细胞内诱导RNAi,抑制人端粒酶催化亚基(human telomerase reverse transcriptase,hTERT)基因表达,在体外细胞水平探讨RNAi对MCF-7乳腺癌细胞生长的影响。 方法:构建靶向hTERT基因的siRNA(hTERT-siRNA),转导入MCF-7乳腺癌细胞,在瘤细胞内诱导RNAi,采用细胞增殖抑制实验、RT-PCR法、Western blot等技术检测siRNA处理前后瘤细胞增殖及hTERT基因表达变化。 结果:hTERT-siRNA对乳腺癌细胞生长抑制率达61.94%;hTERT的mRNA表达降低了61.7%,蛋白表达平均降低了52.8%。 结论:siRNA在体外明显抑制了乳腺癌细胞中hTERT基因的表达和瘤细胞增殖。

     

    Abstract: Objective:To investigate the effect of breast cancer cell growth, we utilized RNAi tragedy to inhibit hTERT gene in MCF-7 cells of breast cancer. Methods:Small intererence RNA (siRNA) homologous to hTERT gene (hTERT-siRNA) was recombined with pGenesil-1 vector, then transfected into MCF-7 cells of breast cancer to induce RNAi,and then hTERT gene expression alterations and tumor cell proliferation in both hTERT-siRNA treatment groups and control groups were detected by MTT assay, RT-PCR and Western blot. Results:The expression of hTERT had been obviously inhibited by siRNA.The inhibition rate of cell proliferation was 61.94%.The mRNA level of hTERT was obviously decreased by 61.7%, and its protein expression reduced by 52.8%. Conclusion:siRNA-hTERT effectively inhibited hTERT gene expression and breast cancer cell proliferation in vitro.

     

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出版历程
  • 收稿日期:  2009-02-12
  • 修回日期:  2009-05-20
  • 刊出日期:  2010-07-24

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